Journal of Crop Breeding

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torkaman S, sofaliyan O, zare N, hasaniyan S. (2021). Investigation of Genetic Diversity of Native Ecotypes of Northwestern Medicinal Plant Mentha longifolia using ISSR Molecular Markers. J Crop Breed. 13(37), 1-10. doi:10.52547/jcb.13.37.1
URL: http://jcb.sanru.ac.ir/article-1-1160-en.html

One of the most important medicinal plants of Iran, Poneh belongs to the mint family. Due to the numerous and very useful medicinal properties of oregano, this plant has attracted many studies. In this study 11 different ecotypes of Mentha longifolia belonging to 11 different regions of Iran including West Azarbaijan, Yazd, Gilan, Chaharmahal Bakhtiari, Khuzestan, Lorestan, Kerman, Hormozgan, Khorasan, Qazvin and Ardabil were studied. In order to investigation genetics variety between pennyroyal ecotypes in Mohaghegh Ardebili University, an experiment was implemented in framework of complete random block in 11 ecotypes (treatments) and 3 repeats. DNA was extracted and evaluated using 23 different ISSR primers to evaluate the genetic diversity among ecotypes. The results indicated sufficient polymorphism among these ecotypes. A total of 60 clear and scalable bands were produced, 60 of which are polymorphic. In p9 and p8 markers based on Shannon’s genetics variety, Ni‘s gene variety and multiple-shaped content had the most highest and lowest values between cases respectively. Also two mentioned markers had most highest and lowest values for effective alleles number. According to Shannon’s and Ni’s markers, Kerman and Yazd population had most highest and lowest values for mentioned two features. Also, based on Ni’s genetic distance; genetics distance between ecotypes is variable from 0.285 in Kerman and Qazvin to 0.672 in Hormozghan and Kerman and 0.625 in Yazd and Qazvin.  Grouping is done based on ISSR markers similarity matrix and ecotypes were classified in 3 cases. In PCA, 2 primitive components describe more than 56% of variance. Existence of genetics variety between pennyroyal ecotypes for goals selection possibility.