Journal of Crop Breeding

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Nazari M, Zaynali Nezhad K, Navabpour S, Soltanlo H, Pahlavani M. (2019). Application of Allele Specific Markers in Determining Alleles at VRN-1 Loci in Bread Wheat Genotypes. jcb. 11(32), 133-143. doi:10.29252/jcb.11.32.133
URL: http://jcb.sanru.ac.ir/article-1-978-en.html

    Wheat is the most important crop and is cultivated all over the world. Wheat has a wide range of adaptability to different climates. Vernalization genes are one of the important factors determining wheat adaptability. in wheat breeding program understanding the alleles at vernalization requirement gens are useful to introduce new cultivar for different climates. At the molecular level, the length of the vernalization period of common wheat (Triticum aestivum L.) is determined mainly by three loci: VRN-1, VRN-2 and VRN-3. The VRNA1, VRN-B1, and VRN-D1 genes are dominant for spring growth habit and epistatic to the alleles for winter growth habit. Therefore, winter cultivars are homozygous for the recessive alleles at the three VRN-1 loci. In the current study, 34 bread wheat accessions plus Chinese Spring wheat were investigated for allelic variation at Vrn1 loci. The current study showed that five genotypes had dominant allele at VRN-A1. VRN-A1b allele was not found at any of the genotypes. Only one genotype had VRN-A1c allele and 32 genotypes including the Chinese Spring showed vrn-A1 allele. The genotype No. 6 unexpectedly had more than one allele. At locus VRN-B, 14 genotypes had dominant allele while 20 including the Chinese Spring showed recessive allele. ele was not found at any of the genotypes. Only one genotype had VRN-A1c allele and 32 genotypes including the Chinese Spring showed vrn-A1 allele. The genotype No. 6 unexpectedly had more than one allele. At locus VRN-B, 14 genotypes had dominant allele while 20 including the Chinese Spring showed recessive allele.