Volume 9, Issue 24 (3-2018)
jcb 2018, 9(24): 103-111 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rahmatinia M, Moradi H, Ghasemi Omran V A, Hadadinejad M. (2018). The Effect of Different Magnetic Field Duration on Direct Organogenesis of African Violets (Saintpaulia Ionantha) In Tissue Culture Medium with and Without Pgrs. jcb. 9(24), 103-111. doi:10.29252/jcb.9.24.103
URL: http://jcb.sanru.ac.ir/article-1-942-en.html
URL: http://jcb.sanru.ac.ir/article-1-942-en.html
Department of Horticultural Sciences, Sari Agricultural Sciences and Natural Resources University
Abstract: (3671 Views)
To study intermittence of duration of exposure to magnetic field or MF (0.9 mT) on direct organogenesis of African violet explant in medium with and without PGRs these experiments performed. First experiment performed as completely randomized design (CRD) with three level of MF exposure (control, 5 day continuous and 5 day discontinuous) in PGR- free medium included via 6 repeat. The second experiment performed as factorial in CRD. Factors included auxin and cytokenin as two PGRs in 1mg Lit and 3mg Lit IBA and BAP levels and 3 levels of MF exposure as well as first experiment with 6 repeat. The results showed that explants exposed to a discontinuous MF with and without PGRs produced the highest shoot number (7.08) and the lowest number was produced in the control (2.35). Plantlets length was not affected by the MF but it was affected inside of PGRs. The leaf number in the continuous (4.4) and discontinuous (4.7) magnetic field was approximately doubled in comparison with control (2.77). The fresh and dry weight revealed better values in the MF discontinuous (0.49 and 0.03 mg) than continuous (0.35 and 0.02 mg) in PGRs-free medium. Results showed the fresh weight of plantlets was significantly higher for 1mgl-1 PGRs than 3 mgl-1 PGRs, when exposed to 0.9 mT MF for 5 days continuously, as well as for dry weight. It seems that the MF as a light abiotic stress can simulate the cell division and elongation and eventually the plant growth.
Type of Study: Research |
Subject:
اصلاح نباتات، بیومتری
Received: 2018/03/10 | Revised: 2019/04/14 | Accepted: 2018/03/10 | Published: 2018/03/10
Received: 2018/03/10 | Revised: 2019/04/14 | Accepted: 2018/03/10 | Published: 2018/03/10
References
1. Abaszadeh, R., M. Masumiyan, S.H. Sarami, A. Zenuzi, A. Nouruziyan and F. Sadatmirsafi. 2014. Study the effects of electromagnetic fields on phenol the amount Production of Aloe vera. In proceedings of The 3th conference of Electromagnetic engineered (COM) Iran, 3-4 Des., Ministry of Science, Research and Technology scientific and industrial research organization in Iran, pp: 1-6 (In Persian).
2. Alikamanoğlu, S., O. Yaycılı, C. Atak and A. Rzakoulieva. 2007. Effect of magnetic field and gama radiation on Paulowinia tomentosa tissue culture. Biotechnology & Biotechnological Equipment, 21, 49-53, Retrieved 30 January 2016, from http://dx.doi.org/10.1080/13102818.2007.10817412. [DOI:10.1080/13102818.2007.10817412]
3. Arbabian, S., A. Majd and S. Salaripour .2010. The effects of electromagnetic field (EMF) on vegetative organs, pollen development, pollen germination and pollen tube growth of Glycine max L. Cell and Tissue Journal, 1: 35-42 (In Persian).
4. Atak, C., O. Çelik, S. Alikamanoğlu and A. Rzakoulieva. 2007. Effect of magnetic field on peroxidase activities of soybean tissue culture. Biotechnology & Biotechnological Equipment, 21(2): 166-171. [DOI:10.1080/13102818.2007.10817438]
5. Atharinia, M., M. Nori and F. Ghanati. 2009. Effect of static magnetic field on certain physiological and biochemical features of Cicer arietinum in vegetative growth phase. Pajouhesh & Sazandegi, 80: 62-74 (In Persian).
6. Çelik, Ö., Ç. Atak and A. Rzakulieva. 2008. Stimulation of rapid regeneration-by a magnetic field in Paulownia node cultures. Journal of Central European Agriculture, 9: 297-30412.
7. Kato, R., H. Kamada and M. Asashma.1989. Effect of high and very low magnetic field on the growth of hairy roots of Daucus carotta and Atropa belladonna. Cell Physiology, 30: 605-608. [DOI:10.1093/oxfordjournals.pcp.a077782]
8. Khoshsokhan-Mozafar, M., F. Ghanati, H. Zare Maivan, P. Abdolmaleki, K.H. Khorrami Shad and M. Vaeszadeh.2006. The effects of static magnetic field on the metabolism of certain phenolic compound in red cabbag (Brassica oleracea L. cv. Staccato). Pajouhesh & Sazandegi, 70: 63-69 (In Persian).
9. Kordas, L. 2002. The effect of magnetic field on growth, development and the yield of spring wheat. Polish Journal of Environmental Studies, 11: 527-530.
10. Liboff, A.R., B.R. McLeod and S.D. Smith.1989. Rotating magnetic fields and iron cyclotron resonance Journal of Bioelectronics, 8: 119-125. [DOI:10.3109/15368378909020949]
11. Paeez, A., F. Ghanati, M. Behmanesh, P. Abdolmaleki and B. Nahidian. 2012. Comparative study on the effect of static and electromagnetic fields on the some physiological properties at vegetative stage of wheat. In: Proceedings of 1th national conference on plant stress (abiotic), Isfahan University, Isfahan, Iran, pp: 1-3.
12. Paul, A., F. Robert and M. Meisel. 2006. High magnetic field induced changes of gene expression in Arabidopsis. Biomagnetic Research and Technology, 14: 103-7.
13. Pazur, A. and V. Rassadina. 2008. Transient effect of weak electromagnetic fields on calcium ion concentration in Arabidopsis thaliana. BMC Plant Biology, Received: 26 November 2008, from http://www.biomedcentral.com/1471-2229/9/47. [DOI:10.1186/1471-2229-9-47]
14. Rajabbeigi, E., F. Ghanati, F. Sefidkon and P. Abdolmaleki. 2007. Investigating the changes of essential oil of Ocimum basilicum L. in response to electromagnetic field. Iranian Journal of medicinal and Aromatic Plants, 22(4): 341-350.
15. Sadeghian, S., Gh. Ranjbar and K. Kazemitabar. 2014. Consideration and selection of suitable Hormonal Composition for in vitro Shoot Regeneration and propagation of Ocimum basilicum L. Journal of1Crop Breeding, 6(13): 40-48 (In Persian).
16. Tahmasbe, A., M.A. Asghari, O. Sofalian, H.R. Mohammaddoust, C. Abad and A. Rasoulzadeh. 2016. Effect of osmotic stress on some physiological characters of wheat cultivars. Journal of Crop Breeding, 8(19): 112-123 (In Persian).
17. Van, P.H., J. Silva, L. Ham and M. Tanaka. 2012. Effects of permanent magnetic fields on in vitro growth of Cymbidium and Spathiphyllum shoots. In Vitro Cellular & Developmental Biology Plant, 48: 225-232. [DOI:10.1007/s11627-012-9423-6]
18. Yaycili, O. and S. Alikamanoglu. 2005. The effect of magnetic field on Paulownia tissue cultures. Plant Cell Tissue Organ Culture, 83: 109-114. [DOI:10.1007/s11240-005-4852-0]
19. Zamiran, A., M.R. Maleki and V.R. Safari. 2011. Effect of magnetic field on seed germination rate of Zinnia. In: Proceedings of 1th national congress of modern science and technology of agriculture, Zanjan University, Zanjan, Iran, pp: 1-4.
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
