Volume 9, Issue 24 (3-2018)
J Crop Breed 2018, 9(24): 186-191 |
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sahraro A, Zarei A, Mirjalili M H, Akbarpoor V, Corchete P. (2018). Effects of Vanadyl Sulfate on Media Consumption by Satureja khuzistanica Cells and Rosmarinic Acid Biosynthesis. J Crop Breed. 9(24), 186-191. doi:10.29252/jcb.9.24.186
URL: http://jcb.sanru.ac.ir/article-1-927-en.html
URL: http://jcb.sanru.ac.ir/article-1-927-en.html
Amir Sahraro *1, Abdolkarim Zarei2, Mohammad Hossien Mirjalili3, Vahid Akbarpoor4, Purificación Corchete5
1- Department of Horticulture, University of Gilan
2- Department of Biotechnology, Jahrom University
3- Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University
4- Department of Horticulture, Sari Agricultural Sciences and Natural Resources University
5- Department of Plant Physiology, Faculty of Biology, University of Salamanca, E-37007 Salamanca
2- Department of Biotechnology, Jahrom University
3- Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University
4- Department of Horticulture, Sari Agricultural Sciences and Natural Resources University
5- Department of Plant Physiology, Faculty of Biology, University of Salamanca, E-37007 Salamanca
Abstract: (4290 Views)
Application of biotechnology for production and enhancing of important secondary metabolite by plant cell cultures and elicitors is one of the most noteworthy researches by scientists. This research was carried out with the aim of analysis the effects of Vanadyl Sulfate (abiotic elicitor) on media consumption by Satureja khuzistanica cells under suspension culture. Cells obtained after four subcultures from suspension culture of Satureja khuzistanica used in the present study. B5 liquid medium containing 5 mg.l-1 BA (benzyl adenine) and 1 mg.l-1 IBA (Indole-3-butyric acid) were prepared. Three levels of Vanadyl Sulfate (0, 2.5 and 5 mg.l-1) were analyzed. According to results, the highest consuming rate of culture media and the lowest cell dry weight obtained by 2.5 mg.l-1 Vanadyl sulfate. Furthermore, concentration of 5 mg.l-1 showed approximately equal content of dry biomass compared to the control condition. The maximum biosynthesis of rosmarinic acid was obtained by cells in media containing 2.5 mg.l-1 Vanadyl sulfate. These results showed that application of Vanadyl sulfate have positive effects on biosynthesis of rosmarinic acid. Altogether application of different concentrations of Vanadyl sulfate as an abiotic elicitor in Satureja khuzistanica showed a normal dry matter production; while rosmarinic acid was increased. Therefore, application of this elicitor in suspension culture of Satureja khuzistanica is suggested to increase rosmarinic acid production.
Type of Study: Research |
Subject:
اصلاح نباتات، بیومتری
Received: 2018/03/10 | Accepted: 2018/03/10
Received: 2018/03/10 | Accepted: 2018/03/10
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