Journal of Crop Breeding

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Strawberry is perennial, herbaceous plants belonging to the family of fragaria, with a rich source of vitamins and minerals. The aim of present study was to evaluate the effects of different concentrations of growth regulators including auxin and cytokinin to identify the best hormonal concentration to obtain the highest yield of in vitro produced plantlets from strawberry cv Diamante. Experiment was performed in a factorial experiment based on a completely randomized design, using the basal MS culture medium. In this experiment the effects of different concentrations of BA (0/2, 0/5, 1 and 2 mg/l) , IBA (0, 0/1 and 0/5 mg/l) and NAA (0, 0/1 and 0/5 mg/l) alone or in combination with each other for regeneration of shoot tip explants and NAA (0/2, 0/5 and 1 mg/l) and IBA (0/2 , 0/5 and 1 mg/l) for rooting were studied alone. Number and length of shoots and roots produced per explants were evaluated. Results showed that the highest number of regeneration obtained from MS medium included 1 mg/l BA. The highest shoot length was found in the combined treatment of BA and IBA (0/5 + 0/5) mg/l.The maximum rooting was observed in the 1/2 MS medium containing 0/2 mg/l NAA and the longest roots obtained from control treatment.

Keywords: Growth regulators, Regeneration, Rooting, Strawberry, Shoot

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