دوره 9، شماره 24 - ( زمستان 1396 )                   جلد 9 شماره 24 صفحات 166-178 | برگشت به فهرست نسخه ها


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Asghari A, mohammadniya S, Fallahi H. Assesment of Salinity Tolerance in Some Canola Cultivars using Morphophysiologic Traites and ISSR Markers. jcb. 2018; 9 (24) :166-178
URL: http://jcb.sanru.ac.ir/article-1-931-fa.html
اصغری علی، محمدنیا شیوا، فلاحی هاجر. بررسی تحمل به شوری در برخی ارقام کلزا با استفاده از صفات مورفوفیزیولوژیک و نشانگرهای ISSR . پژوهشنامه اصلاح گیاهان زراعی. 1396; 9 (24) :166-178

URL: http://jcb.sanru.ac.ir/article-1-931-fa.html


دانشگاه محقق اردبیلی
چکیده:   (1370 مشاهده)
کلزا (Brassica napus L.) یکی از مهم­ترین گیاهان روغنی جهان محسوب می­شود. از طرفی خاک­های شور و شوری آب آبیاری از مهم­ترین عوامل تنش­زای محیطی در تولید کلزا می­باشند. به­منظور بررسی تحمل به شوری ارقام کلزا در مرحله گیاهچه از لحاظ صفات مورفوفیزیولوژیک و بررسی ارتباط این صفات با نشانگر­های ISSR، 15 رقم کلزا در دو سطح تنش شوری 150 میلی­مولار و 250 میلی­مولار و شرایط بدون تنش به­صورت آب کشت در شرایط گلخانه­ای مورد بررسی قرار گرفتند. در این مطالعه تنش شوری موجب کاهش اکثر صفات مورد ارزیابی در ارقام کلزا به­جز محتوای سدیم و نسبت سدیم به پتاسیم شد. نتایج مقایسه میانگین و تجزیه­ خوشه­ای نشان­داد که رقم­های SLMO46 و PF از نظر اکثر صفات در شرایط مختلف مورد بررسی نسبت به بقیه ارقام برتر بوده در حالی­که، ارقام Hayola60 و Licord کمترین مقدار را به­خود اختصاص دادند. در بررسی مولکولی با استفاده از 11 آغازگر ISSR، 45 نوار چندشکل در ارقام مورد مطالعه کلزا تولید شد. میانگین محتوای اطلاع چندشکلی و شاخص نشانگری برای کلیه آغازگر­ها به­ترتیب 282/0 و 108/1 به­دست آمد. در تجزیه خوشه­ای با استفاده از داده­های ISSR و فاصله ژنتیکی نی، ارقام کلزا به سه گروه تقسیم شدند. کمترین فاصله ژنتیکی بین دو رقم Zarfam و Jewel (079/0) و بیشترین فاصله بین ارقام Quantum و Hyola60 با SLMO46 (32/0) به دست آمد.
متن کامل [PDF 723 kb]   (969 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: اصلاح نباتات، بیومتری
دریافت: 1396/12/19 | ویرایش نهایی: 1398/1/25 | پذیرش: 1396/12/19 | انتشار: 1396/12/19

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