Volume 11, Issue 29 (3-2019)
jcb 2019, 11(29): 127-133 |
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Ahmadi-Ochtapeh H, Soltanloo H, Ramezanpour S S, Yamchi A, Shariati V. (2019). Relationship between Improvement of the Baking Quality and Down-Regulation of Dx2 and Dy12 Genes in Mutant Bread Wheat . jcb. 11(29), 127-133. doi:10.29252/jcb.11.29.127
URL: http://jcb.sanru.ac.ir/article-1-777-en.html
URL: http://jcb.sanru.ac.ir/article-1-777-en.html
Hossein Ahmadi-Ochtapeh 
, Hassan Soltanloo , Sayede Sanaz Ramezanpour , Ahad Yamchi , Vahid Shariati
, Hassan Soltanloo , Sayede Sanaz Ramezanpour , Ahad Yamchi , Vahid Shariati
Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), IRAN
Abstract: (3022 Views)
In current research, the expression level of Dx2 and Dy12 genes on Glu-D1 locus that encoding the high-molecular-weight glutenin subunits (HMW-GSs), with negative impact on quality of bakery in genotype mutant bread wheat called RO-3 with high quality of bakery and its parent (Roshan) with low quality of bakery was investigated. For this purpose, sampling was performed grains at intervals of 5, 10, 15, 20 and 30 days post-anthesis (DPA). The gene expression results showed that RO-3 genotype had the highest gene expression decreasing for Dy12 genes at 5 DPA compared to parental genotype, and for Dx2 gene the highest gene expression decreasing was 10 DPA compared to parental genotype. Generally, during seed development both genes in mutant genotype had gene expression decreasing than the parental genotype. The most dry weight accumulation rates occurred in 10 to 15 DPA in mutant genotype. So, the early stages of seed development for reducing the expression of these genes and rates dry weight accumulation is critical in mutant genotype. The grain-filling rate and the maximum grain weight were higher in mutant genotype than the parental genotype. Therefore breeding the cultivars with higher grain filling rate and also selection of varieties with low expression for Dx2 and Dy12 genes play important role in the selection of varieties with high yield and quality of bakery.
Keywords: Gene expression, Glutenin heavy subunit, QRT-PCR, Quality of bakery, Seed development, Wheat
Type of Study: Research |
Subject:
اصلاح موتاسيوني
Received: 2017/06/14 | Revised: 2019/05/14 | Accepted: 2017/09/10 | Published: 2019/05/8
Received: 2017/06/14 | Revised: 2019/05/14 | Accepted: 2017/09/10 | Published: 2019/05/8
References
1. Ahmad, M. 2000. Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers. Theoretical and Applied Genetics, 101: 892-896. [DOI:10.1007/s001220051558]
2. Altenbach, S.B and K.M. Kothari. 2004. Transcript profiles of genes expressed in endosperm tissue are altered by high temperature during wheat grain development. Journal of Cereal Science, 40: 115-126. [DOI:10.1016/j.jcs.2004.05.004]
3. Altenbach, S.B., K.M. Kothari and D. Lieu. 2002. Environmental conditions during wheat grain development alter temporal regulation of major gluten protein genes. Cereal Chemistry, 79: 279-285. [DOI:10.1094/CCHEM.2002.79.2.279]
4. Barak, S., D. Mudgil and B.S. Khatkar. 2013. Relationship of gliadin and glutenin proteins with dough rheology, flour pasting and bread making performance of wheat varieties. LWT - Food Science and Technology, 51: 211-217. [DOI:10.1016/j.lwt.2012.09.011]
5. Brdar, M.D., M.K.B. Marija and D. Borislav. 2008. The parameters of grain filling and yield components in common wheat (Triticum aestivum L.) and durum wheat (Triticum turgidum L. Var.Durum.) Central European Journal of Biology, 3(1): 75-82. [DOI:10.2478/s11535-007-0050-x]
6. Campos, H., J. Zuniga, P. Rathgeb and C. Jobet. 2004. Selection of Chilean wheat genotypes carriers of the HMW glutenin allele Glu-D1 x5 through polymerase chain reaction. Agricultura Técnica, 64: 223-228. [DOI:10.4067/S0365-28072004000300001]
7. Dupont, F.M., W.J. Hurkman, W.H. Vensel, C. Tanaka, K.M. Kothari, O.K. Chung and S.B. Altenbach. 2006. Protein accumulation and composition in wheat grains: effects of mineral nutrients and high temperature. European Journal of Agronomy, 25: 96-107. [DOI:10.1016/j.eja.2006.04.003]
8. Ellis, R.H and C. Pieta-Filho. 1992. The development of seed quality in spring and winter cultivars of barley and wheat. Seed Science Research, 2: 19-25. [DOI:10.1017/S0960258500001057]
9. Ferreira, M.S.L., J. Bonicel, N.N. Rosa, M.F. Samson and M.H. Morel. 2009. How is gluten polymers assembled during grain filling in durum wheat? Proceedings of the 10th International Gluten workshop, 29-33.
10. Hghparast, R., R. Rajabi, G. Najafiyan, K. Rashmekarim and M. Aghayi Sarbarzeh. 2009. Evaluation of Indices Related to Grain Quality in Advanced Bread Wheat Genotypes under Rainfed Conditions. Seed and Plant Improvement Journal, 25(2): 315-328 (In Persian).
11. Izadi-Darbandi, A., B. Yazdi-Samadi, A.A. Shanejat-Boushehri and M. Mohammadi. 2010. Allelic variations in Glu-1 and Glu-3 loci of historical and modern Iranian bread wheat (Triticum aestivum L.) cultivars. Journal of Genetics, 89(2): 193-199. [DOI:10.1007/s12041-010-0025-y]
12. Karataş, D.D., B. Kunter, G. Coppola and R. Velasco. 2010. Analysis of polymorphism based on SSCP markers in gamma-irradiated (Co60) grape (Vitis vinifera) varieties. Genetics and Molecular Research, 9(4): 2357-2363. [DOI:10.4238/vol9-4gmr864]
13. Kiani, D., H. Soltanloo, S.S. Ramezanpour, A.A. Nasrolahnezhad Qumi, A. Yamchi, K. Zaynali Nezhad and E. Tavakol. 2017. A barley mutant with improved salt tolerance through ion homeostasis and ROS scavenging under salt stress. Acta Physiol Plant, 39(90): 1-14. [DOI:10.1007/s11738-017-2359-z]
14. Kumari, S.L and G. Valarmathi. 1998. Relationship between grain yield grain filling rate and duration of grain filling in rice. Madras Agricultural Journal, 85: 210-211.
15. Lei, Z.S., K.R. Gale, Z.H. He, C. Gianibelli, O. Larroque, X.C. Xia, B.J. Butow and W. Ma. 2006. Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat. The Journal of Cereal Science, 43: 94-101. [DOI:10.1016/j.jcs.2005.08.003]
16. Liu, S., X. Gao and G. Xia. 2009. Characterizing HMW-GS alleles of decaploid Agropyron elongatum in relation to evolution and wheat breeding. Theoretical and Applied Genetics, 116: 325-334. [DOI:10.1007/s00122-007-0669-z]
17. Md Zaidul, I.S., A. Abd Karim, D.M.A. Manan, A. Ariffin, N.A. Nik Norulaini and A.K. Mohd Omar. 2004. farinograph study on the viscoelastic properties of sago/wheat flour dough systems. Journal of the Science of Food and Agriculture, 84: 616-622. [DOI:10.1002/jsfa.1713]
18. Moradi, M., and M. Motamedi. 2010. Speed and duration of grain filling in some wheat cultivars. Quarterly Journal of Plant Production Science (Journal of Agricultural), 4: 37-43 (In Persian).
19. Najafiyan, G., and N. Baghayi. 2011. Genetic Variation in High Molecular Weight Glutenin Subunits in Parental Lines and Cultivars of Wheat Used in Breeding Programs of Cold and Temperate Agro - Climatic Zones of Iran. Seed and Plant Improvement Journal, 27(3): 305-322 (In Persian).
20. Payne P.I., M.A. Nigtingale, A.F. Krattiger and L.M. Holt. 1987. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. Journal of the Science of Food and Agriculture, 40: 51-65. [DOI:10.1002/jsfa.2740400108]
21. Pfaffl, M.W., 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29: 2002-2007. [DOI:10.1093/nar/29.9.e45]
22. Pfaffl, M.W., G.W. Horgan and L. Dempfle. 2002. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real time PCR. Nucleic Acids Research, 30: 36-46. [DOI:10.1093/nar/30.9.e36]
23. Sarkar, S., A.M. Singh, A.K. Ahlawat, M. Chakraborti and S.K. Singh. 2015. Genetic diversity of bread wheat genotypes based on High Molecular Weight Glutenin Subunit profiling and its relation to bread making quality. Journal of Plant Biochemistry and Biotechnology, 24(2): 218-224. [DOI:10.1007/s13562-014-0261-y]
24. Shewry P.R., N.G. Halford and A.S. Tatham. 1992. High-molecular weight subunits of wheat glutenin subunits of wheat glutenin. Journal of Cereal Science, 5: 105-120. [DOI:10.1016/S0733-5210(09)80062-3]
25. Singh, N.K. and H.S. Balyan. 2009. Induced mutations in bread wheat (Triticum aestivum L.) CV. 'Kharchia 65' for reduced plant height and improves grain quality traits. Advances in Biological Research, 3(5-6): 215-221.
26. Tabiki T., S. Ikeguschi and T.M. Ikeda. 2006. Effects of High-molecular-weight and Low-molecular-weight glutenin subunit alleles on common wheat flour quality. Breeding Science, 56: 131-136. [DOI:10.1270/jsbbs.56.131]
27. Van Sanford, D.A. 1985. Variation in kernel growth characters among soft red winter wheat. Crop Science, 25: 625-630. [DOI:10.2135/cropsci1985.0011183X002500040012x]
28. Zamani, M.J., M.R. Bihamta, B. Naseriyan Khiyabani and M.T. Halajiyan. 2007. Selection of bread wheat mutant genotypes carrying HMW glutenin alleles related to baking quality through sequence tagged site. Journal of Agronomy and Plant Breeding, 1(3): 63-71 (In Persian).
29. Zamani, M.J., M.R. Bihamta, B. Naserian Khiabani, Z. Tahernezhad, M.T. Hallajian and M. Varasteh Shamsi. 2014. Marker-Assisted Selection for Recognizing Wheat Mutant Genotypes Carrying HMW Glutenin Alleles Related to Baking Quality. The Scientific World Journal, Article ID 387912, 5 pp. [DOI:10.1155/2014/387912]
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