Volume 10, Issue 28 (12-2018)
J Crop Breed 2018, 10(28): 101-109 |
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daneshvar Z, saeidi A, Beyrami Zadeh E. (2018). Comparison of the Performance of SCoT and RAPD Markers for Study of Genetic Diversity and Relationships of Anthurium (Anthurium andreanum) Cultivars. J Crop Breed. 10(28), 101-109. doi:10.29252/jcb.10.28.101
URL: http://jcb.sanru.ac.ir/article-1-775-en.html
URL: http://jcb.sanru.ac.ir/article-1-775-en.html
1- Department of Science and Biotechnology, Faculty of Biotechnology, Shahid Beheshti University, Tehran,
2- Department of Genetics and Rape, Department of Flowers and Ornamental Plants, Institute of Horticulture, Research, Education and Promotion Center of Mahallat
2- Department of Genetics and Rape, Department of Flowers and Ornamental Plants, Institute of Horticulture, Research, Education and Promotion Center of Mahallat
Abstract: (3058 Views)
Survey of genetic diversity is essential for breeding program. The genetic diversity of A. andreanum, 10 cultivars were analyzed using seven Start Codon Targeted (SCoT) and 40 RAPD markers in which 22 RAPD markers and all of SCoT marker produced polymorphic bands. Matrix genetic distance ranged from 0.01 to 0.37 for RAPD and from 0.02 to 0.52 for SCoT marker analysis. Polymorphism percentage generated by SCoT markers (100%) was similar to RAPD markers. In order to determine genetic relationships, cluster analysis was performed using Neighbor-joining with Splits tree software. Cluster analysis for RAPD and SCoT markers revealed that cultivars were grouped in three clusters. Results from the present study revealed the superiority of SCoT over RAPD markers due to their high resolution and high variability in detection of genomic information. This is the first report of using SCoT marker for genetic diversity analysis in anthorium. Overall, results showed that these markers provide useful information to protect A. andreanum germplasm resources and also useful for breeding programs.
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