دوره 10، شماره 27 - ( پاییز 1397 )
جلد 10 شماره 27 صفحات 83-75 |
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Parsamanesh Z, Hedayat M, Bayat F. (2018). Effects of Different Media Factors on In Vitro Proliferation of St. John’s Wort (Hypericum perforatum). J Crop Breed. 10(27), 75-83. doi:10.29252/jcb.10.27.75
URL: http://jcb.sanru.ac.ir/article-1-746-fa.html
URL: http://jcb.sanru.ac.ir/article-1-746-fa.html
پارسامنش زینب، هدایت محمد، بیات فرشته. تأثیر فاکتور های مختلف محیط کشت بر پرآوری درون شیشه ای گل راعی (Hypericum perforatum) پژوهشنامه اصلاح گیاهان زراعی 1397; 10 (27) :83-75 10.29252/jcb.10.27.75
1- دانشگاه خلیج فارس
چکیده: (3435 مشاهده)
گل راعی از گیاهان دارویی مهم بوده که از قدیم در درمان بیماریهای عصبی و روانی مورد استفاده قرار می گرفت. این پژوهش جهت بهینه نمودن محیط کشت و انتخاب ریزنمونه مناسب جهت تسریع ریشه زایی و باززایی گیاه صورت گرفت. جهت باززایی و پرآوری از دو ریزنمونه شامل شاخساره با یک و دو گره در محیط کشت MS شامل سه سطح MSکامل و باغلظت های 30 و 45 گرم بر لیتر ساکاروز و تنظیم کننده رشدBA با غلظتهای 05/0، 1/0، 2/0 میلیگرم بر لیتر استفاده شد. بهترین محیط کشت پرآوری گل راعی در ریزنمونه یک گره با محیط کشت حاوی 30 گرم در لیتر ساکاروز به همراه 1/0 میلی گرم بر لیتر BA به دست آمد. همچنین کاهش غلظت محیط کشت MS و ساکاروز با میزان متوسط غلظت BA موجب افزایش تعداد شاخساره ها گردید. به منظور تولید ریشه از سطوح مختلف تنظیم کننده رشد IBA با غلظت های صفر، 2/0، 4/0، 6/0، 8/0 و 1 میلیگرم بر لیتر استفاده شد. نتایج نشان داد ریزنمونه ها در گیاه گل راعی برای تولید ریشه نیازی به تنظیم کننده های رشد بیرونی ندارند، اما افزودن IBA و افزایش غلظت آن موجب افزایش طول و تعداد ریشه ها شد.
فهرست منابع
1. Abou Dahab, A.M., M.A. Afaf, Y.A. HabibHosni and A.M.M. Gabr. 2005. Effect of MS-salt strength, sucrose and IBA concentration and acclimatization media on Ruscus hypoglossum L. micropropagation. Arab Journal of Biotechnology, 8(1): 141-154.
2. Akbas, F., C. Isikalan, S. Namli, P. Karakus and D. Basaran. 2011. Direct plant regeneration from in vitro derived leaf explants of Hypericum spectabile, a medicinal plant. Journal of Medicinal Plants Research, 5(11): 2175-2181.
3. Ayan, A.K., C. Cirak, K. Kevserolu and A. Sokmen. 2005. Effects of expellant types and different concentrations of sucrose and phytoharmones on plant regeneration and hypericin content in Hypericum perforatum L. Turkish Journal of Agriculture, 29: 197-204.
4. Banerjee, A., S. Bandyopadhyay and S.S. Raychaudhuri. 2012. In vitro regeneration of Hypericum perforatum L. using thidiazuron and analysis of genetic stability of regenerates. Indian Journal of Biotechnology, 11: 92-98.
5. Barnes, J., A. Anderson and D. Phillipson. 2001. St. John's wort (Hypericum perforatum L.): A review of its chemistry, pharmacology and clinical properties. Journal of pharmacy and pharmacology, 53: 583-600. [DOI:10.1211/0022357011775910]
6. Bidarigh, S. and E. Azarpour. 2013. Study effect of MS medium and shoot tip length levels on rooting in micro cuttings of poinsettia. Intl. Journal of Agricultural Crop Science, 5(8): 857-860.
7. Bourgaud, F., A. Gravot and E. Goniter. 2002. Production of plant secondary metabolites. Plant Science, 161: 839-851. [DOI:10.1016/S0168-9452(01)00490-3]
8. Carolina, A., L.V. Astarita and E.R. Santarem. 2001. Organogenese in Hypericum perforatum L. IV Encontro Latino-Americano de Biotechnologia Vegetal, Goiania, Resumos, 81 pp.
9. Cirak, C., A.K. Ayan and K. Kevseroglu. 2007. Direct and indirect regeneration of plants from inter nodal and leaf explants of Hypencum bupleuroides Gris. Journal of Plant Biology, 50(1): 24-27. [DOI:10.1007/BF03030596]
10. Ebrahimi, F. 2010. Effect of different hormonal concentrations and culture medium on eglantine (Rosa foetida) propagation in tissue culture medium. Plant Ecophysiology, 2: 145-149.
11. Elyasi, L., A.A. Mehrabi, M. Seyedi and Z. Safari. 2016. Optimization of Callus Culture of Satureja Bachtiarica L. using Different Explants and Concentrations of Growth Regulators. Journal of Crop Breeding. 8(20): 124-132 (In Persian).
12. Franklin G. and A.C.P. Dias. 2006. Organogenesis and embryogenesis in several Hypericum Perforatum genotypes. In Vitro Cellular and Development Biology- Plant, 42: 324-330. [DOI:10.1079/IVP2006787]
13. Franz, C.H. 1983. Nutrient and water management for medicinal and aromatic plant. Acta Horticulture, 132: 203-215. [DOI:10.17660/ActaHortic.1983.132.22]
14. Ghazian Tafrishi, G., M. Azizi and M. Farsi. 2006. Investigation of in vitro Culture of Iranian St. John's Wort (Hypericum perforatum L.). Iranian Journal of Medicinal and Aromatic Plants, 22(3): 172-179 (In Persian).
15. Goel, M.K., A.K. Kukreja and N.S. Bisht. 2009. In vitro manipulations in St. John's wort (Hypericum perforatum L.) for incessant and scale up micropropagation using adventitious roots in liquid medium and assessment of clonal fidelity using RAPD analysis. Plant Cell Tissue and Organ Culture, 96: 1-9. [DOI:10.1007/s11240-008-9453-2]
16. Groll, J., D.J. Mycock and V.M. Gray. 2002. Effect of medium salt concentration on differentiation and maturation of somatic embryos of cassava (Manihot esculenta Crantz). Annals of Botany, 89(5): 645-648. [DOI:10.1093/aob/mcf095]
17. Kazemiani, S., A. Motallebi-Azar, N. Mohaddes, F. Kiomarsy, F. Yarmohammdi and F. Etedali. 2012. Effect of different concentrations of sucrose and BAP on shoot proliferation on MS strength basal media in potato cv. Agria. South Western Journal of Horticulture, Biology and Environment, 1(3): 63-72.
18. Khosh-Khui, M., A. Shekafandeh and H. Azarakhsh. 1984. Micropropagation of myrtle. Scientia Horticulturae, 22: 139-146. [DOI:10.1016/0304-4238(84)90094-3]
19. Kubin, A., F. Wierrani, U. Burner, G. Alth and W. Grunberger. 2005. Hypericin the facts about a controversial agent. Current Pharmaceutical Design, 11: 233-253. [DOI:10.2174/1381612053382287]
20. Majd, A., F. Chamandoosti, S. Mehrabian and M. Shedai. 2006. Somatic embryogenesis and plant regeneration in Canola (Brassica napus L.). Iranian Journal of Biology, 20(4): 344-352.
21. Mantell, S. and S. Hugo. 1989. Effects of photoperiod, mineral medium strength, inorganic ammonium, sucrose and cytokinin on root, shoot and microtuber development in shoot cultures of Dioscorea alata L. and D. bulbifera L. yams. Plant Cell, Tissue and Organ Culture, 16: 23-37. [DOI:10.1007/BF00044069]
22. McCoy, J.A. and N.D. Camper. 2002. Development of a Micropropagation Protocol for St. John's Wort (Hypericum perforatum L.). HortScience, 37(6): 978-980. [DOI:10.21273/HORTSCI.37.6.978]
23. Meyer, E.M., D.H. Touchell and T.G. Ranney. 2009. In Vitro Shoot Regeneration and Polyploid Induction from Leaves of Hypericum Species. HortScience, 44(7): 1957-1961. [DOI:10.21273/HORTSCI.44.7.1957]
24. Morshedloo, M.R., E. Ebadi, M.R. Fatahi Moghdam and D. Yazdani. 2012. Explore the components of the oils three plant species Hypericum (Hypericum spp.) In Iran. Journal of Medicinal Plants, 2(9): 23-31 (In Persian).
25. Oluk, E.A., S. Orhan, O. Karaka, A. Cakır and A. Gonuz. 2010. High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hyperıcum trıquetrıfolıum Turra. African Journal of Biotechnology, 9(15): 2229-2233.
26. Pasqual, M., E. Hoshika and J. Susumu Ishida. 1994. Effects of different sucrose and mineral salt concentrations on in vitro propagation of Nephrolepis exaltata: An ornamental fern, Pesquisa Agropecuaria Brasileira, 29(11): 1681-1684.
27. Pretto, R.F. and R.E. Santarem. 2000. Callus formation and plant regeneration from Hypericum perforatum leaves. Plant Cell, Tissue and Organ Culture, 62(2): 107-113. [DOI:10.1023/A:1026534818574]
28. Piri Zirkouhi, M., K. Mashayekhi, B. Kamkar, Kh. Hemmati and F. Vahdatpour. 2009. Embryogenesis of a commercial and a native tomato cultivar using different culture media. Journal of Plant Production, 16(1): 101-114 (In Persian).
29. Sadeghian, S., G.A. Ranjbar and S.K. Kazemitabar. 2014. Consideration and selection of suitable hormonal composition for in vitro shoot regeneration and propagation of Ocimum basilicum L. Journal of Crop Breeding, 6(13): 40-48 (In Persian).
30. Taheri, S.M., H. Daneshvar, M.R. Salehi Salimi and S. Jafari. 2014. The effect of different concentrations of MS medium and growth regulators on seed germination and callus marigold (Calendula officinalis L.) in vitro. First National Congress Iran flowers and ornamental plants. Karaj. National Institute Iran flowers and ornamental plants.
31. Trigiano, R.N. and D.J. Gary. 2005. Plant development and biotechnology. CRC press, Taylor and Francis Group, 376 pp.
32. Tripathi, L. and J.N. Tripathi. 2003. Role of biotechnology in medicinal plants. Tropical Journal of Pharmaceutical Research, 2: 243-253. [DOI:10.4314/tjpr.v2i2.14607]
33. Zamanifar, M., F. Nazarian Firouzabadi and A. Ismaili. 2016. Comparative Study of two Different Cytokinins on Direct Regeneration of Different Sugar beet Explants in Tissue Culture Condition. Journal of Crop Breeding, 8(19): 203-208 (In Persian).
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