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Persian Gulf University
Abstract:   (14 Views)

St. John’s wort is a traditional medicinal plant that has been used from ancient time for the treatment of neurological and psychiatric diseases. This experiment was conducted to optimize the culture medium and selection of appropriate explants to accelerate regeneration and rooting. For regeneration and proliferation of two types of explants, including shoot with one and two nodes in the MS media including three levels of MS full, 3/4 MS and 2/3 MS, with concentrations of 30 and 45 gl-1 of sucrose and BA growth regulators with concentrations of 0.05, 1.0, 2.0 mgl-1 was used. The best proliferation of St. John’s wort was obtained in a single node explant culture medium containing 2/3 MS with 30 gl-1 of sucrose and 0.1mgl-1 BA. Moreover, the results indicated that using the optimum amount of BA with reduced concentrations of sucrose and MS led to increase in the number of shoots. Different levels of growth regulator of IBA with the concentration of zero, 0.2, 0.4, 0.6, 0.8 and 1 mgl-1 were used for production of roots. The results showed that St. John’s wort explant don’t need external growth regulators for the production of root, but addition of IBA and rising in its concentration increases the length and number of roots.

     
Type of Study: Research | Subject: بيوتكنولوژي گياهي

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