Journal of Crop Breeding

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Akbarzadeh Lelekami M, Pahlevani M H, Zaynali Nezhad K, Mahdavi Mashaki K, P.M. Weber A, Brilhaus D. (2020). Response of some of Primary Metabolites in Rice (Oryza sativa L.) Root to Salinity Stress. J Crop Breed. 12(34), 210-217. doi:10.29252/jcb.12.34.210
URL: http://jcb.sanru.ac.ir/article-1-1118-en.html

   In order to investigate the metabolic responses of rice root under salinity stress at seedling stage, an experiment was conducted at greenhouse with five replications as factorial in a completely randomized design including two salinity levels (0 and 150 mM), two sampling times (6h and 54h) and two genotypes CSR28 (tolerant) and IR28 (susceptible). In total, GC-MS analysis identified 36 primary metabolites (including 18 amino acids, five sugars and sugar alcohols and 13 organic acids). The amino acids showed the highest accumulation in facing to high salinity. Moreover, the genotypes showed more differences after the long-term of salinity treatment and the tolerant genotype CSR28 had the highest accumulation of amino acids suggesting the role of osmotic adjustment mechanisms on inducing of salt-tolerance. Among the sugars, raffinose and myoinositol had more accumulation specifically in the tolerant genotype at 54h time point, indicating their antioxidant activity. Generally, these findings emphasized the importance of identification and dissection of metabolic pathways for understanding of the salt-tolerance molecular mechanisms and consequently improving the development of salt-tolerant varieties in rice.