Journal of Crop Breeding

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Olyaei Z, Asghari Zakaria R, Zare N, Berenji D. (2020). Effect of Different Plant Growth Regulators on Callus Induction and Regeneration of Papaver fugax Poir.. jcb. 12(33), 192-201. doi:10.29252/jcb.12.33.192
URL: http://jcb.sanru.ac.ir/article-1-1104-en.html

     Papavear fugax Poir. from Meconidium section of Papaver is an important source for alkaloids. Given the presence of thebain, morphine and codeine in this plant, biotechnological studies, especially In vitro culture of this plant would be of importance. In this study, callus induction and In vitro regeneration of this species from leaf explants in MS medium containing different levels of naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), Kinetin (Kin) and Benzyl adenine purine (BAP) was investigated at 25 °C and 16 h light and 8 h darkness  based on a completely randomized design with three replications. Analysis of variance showed that for callus induction rate and callus weight, there were significant (p<0.01) differences between different culture media. Based on results, the proper medium for callus induction of leaf explants was MS medium supplemented with 1 mg/l NAA and 1 or 0.5 mg/l BAP. The highest callus fresh weight was obtained in MS medium supplemented with 1 mg/l NAA and 1 mg/l BAP. In vitro regeneration of this species from leaf explants was also investigated in MS medium containing different levels of NAA, Kin and BAP and the best medium for regeneration was MS medium supplemented with 1 mg/l Kin and 1 mg/l NAA. By inspecting rooting of seedlings in MS medium containing different levels of indole acetic acid (IAA) plus Kin or BAP, the highest rooting percentage was obtained in MS medium containing 1 mg/l of each of IAA and Kin.