Volume 2, Issue 5 (5-2010)                   jcb 2010, 2(5): 17-29 | Back to browse issues page

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nzyme Assay of Aeluropus littoralis Parl. Regarding to the Salt (NaCl) Stresses. jcb. 2010; 2 (5) :17-29
URL: http://jcb.sanru.ac.ir/article-1-117-en.html
Abstract:   (7734 Views)
Reactive oxygen species (ROS) release from cellular water molecules under abiotic and biotic stresses which are harmful for cellular metabolism. Although, some of radical injuries neutralize via plant enzymatic and nonenzymatic reactions like antioxidant system such as superoxide dismutase, catalase, peroxidase and ascorbate peroxidase. Aeluropus littoralis Parl. is a halophyte plant belongs to Poaceae family can tolerate over 600 mM NaCl stress, thus it can be as genetic source for transfer gene to crop resulting cereal improvement under salt stress condition. There is not any report about antioxidant assay in this plant up to now. This research have studied in rice and citrus research institute, Sari Agricultural Sciences and Natural Resources University’s hydroponic labs in 1389. Antioxidant enzymes assay have performed in four levels, 0, 200, 400 and 600 mM NaCl during 21 days and periodically 72 hours sampling. Statistical analysis indicated reciprocal effects of time and salt treatment for enzyme was significant (α= 0.01). The results determined the enzymes activity increase up to 8, 7, 18 and 5.3 for POX, CAT, APX and SOD respectively in 400 and 600 mM NaCl treatment. In respect to results as well as antioxidant enzymes high activity, identification, isolation and transfer of genes involve in salt tolerance from Aeluropus littoralis Parl. can be benefit for production of transgenic plant tolerant to environmental stresses.
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Type of Study: Research | Subject: General
Received: 2013/05/8 | Published: 2010/05/15

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