Volume 11, Issue 29 (3-2019)                   jcb 2019, 11(29): 9-16 | Back to browse issues page


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Ghasemi N, Mirfakhraii R G, Abbasi A. Assessment of Genetic Diversity of Bread Wheat (Triticum aestivum L.) Cultivars using Microsatellite Markers. jcb. 2019; 11 (29) :9-16
URL: http://jcb.sanru.ac.ir/article-1-770-en.html
Tarbiat Modares University
Abstract:   (672 Views)
Determining the level of genetic diversity and relationships among genotypes is foundation for identifying the appropriate parents in different breeding objects. In present study, the genetic diversity of 22 wheat cultivars were tested using 22 pairs of SSR primers. Out of 24 detected alleles, 11 were able to show desirable polymorphism. In average 2.18 alleles per locus were generated, in which differed from 2 to 3 alleles in each locus. The average of 0.573 polymorphism information content were achieved that Xgwm60 for having high content of polymorphism, was the most appropriate marker for studying genetic diversity in this study. Similarity coefficient was formed with Dice similarity matrix and genotypes were classified into 4 groups. Principal component analysis confirms most of genetic diversity feature derived from cluster analysis. Results of current study showed the vast range of genetic diversity among wheat cultivars that illustrating their possible use in breeding programs.
Full-Text [PDF 1102 kb]   (213 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/05/30 | Revised: 2019/05/14 | Accepted: 2018/09/3 | Published: 2019/05/8

References
1. Aalami, A. and N. Karami. 2017. The study of genetic diversity in Iranian rice cultivars using ISSR, IRAP and REMAP markers. Journal of Crop Breeding, 8(20): 41-51 (In Persian).
2. Agrama, H.A.A. and M.A.R. Tunistra. 2003. Phylogenetic diversity and relationships among sorghum accessions using SSRs and RAPDs. African Journal of Biotechnology, 2(10): 334-340. [DOI:10.5897/AJB2003.000-1069]
3. Asadi, A.A. and S. Rashidi Monfared. 2014. Characterization of EST-SSR markers in durum wheat EST library and functional analysis of SSR-containing EST fragments. Molecular Genetics and Genomics, 289(4): 625-640. [DOI:10.1007/s00438-014-0839-z]
4. Asghari, S. and R.G. Mirfakhraii. 2011. Study on genetic variation in some wheat cultivars using microsatellite markers and physiological traits under late spring stress. M.Sc. Thesis, Tarbiat Modares University, Tehran, Iran, 120 pp (In Persian).
5. Bahraei, S. 2003. Bread wheat quality evaluation based on the high molecular weight Glutenin subunits. Agrobreed, 5(3): 204-215 (In Persian).
6. Bryan, G.J., A.J. Collins, P. Stephenson, A. Orry, J.B. Smith and M.D. Gale. 1997. Isolation and characterization of microsatellites from hexaploid bread wheat. Theoretical and Applied Genetics, 94(5): 557-563. [DOI:10.1007/s001220050451]
7. Christiansen, M.J., S.B. Andersen and R. Ortiz. 2002. Diversity changes in an intensively bred wheat germplasm during the 20th century. Molecular Breeding, 9(1): 1-11. [DOI:10.1023/A:1019234323372]
8. Gupta, P.K. and R.K. Varshney. 2000. The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica, 113(3): 163-185. [DOI:10.1023/A:1003910819967]
9. Gupta, P.K., R.K. Varshney, P.C. Sharma and B. Ramesh. 1999. Molecular markers and their applications in wheat breeding. Plant Breeding, 118(5): 369-390. [DOI:10.1046/j.1439-0523.1999.00401.x]
10. Gupta, P., H. Balyan, K. Edwards, P. Isaac, V. Korzun, M. Röder, M.F. Gautier, P. Joudrier, A. Schlatter, J. Dubcovsky, R. De la Pena, M. Khairallah, G. Penner, M. Hayden, P. Sharp, B. Keller, R. Wang, J. Hardouin, P. Jack and P. Leroy. 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theoretical and Applied Genetics, 105: 413-422. [DOI:10.1007/s00122-002-0865-9]
11. Hedayati Marzoni, H. and H. Samizadeh Lahiji. 2016. Genetic diversity assessment of lines and varieties in winter rapeseed (Brassica napus L.) using RAPD and SSR molecular markers. Journal of Crop Breeding, 8(17): 139-131 (In Persian). [DOI:10.18869/acadpub.jcb.8.17.139]
12. Hokanson, S.C., A.K. Szewc-McFadden, W.F. Lamboy and J.R. McFerson. 1998. Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a malus × domestica borkh core subset collection. Theoretical and Applied Genetics, 97: 671-683. [DOI:10.1007/s001220050943]
13. Khoramipor, S., M. Dehdari and R. Amiri Fahliani. 2017. Study of genetic diversity in sorghum (Sorghum Bicolar L.) genotypes using microsatellite markers. Journal of Crop Breeding, 8(20): 116-123 (In Persian).
14. Liu, J., L. Liu, N. Hou, A. Zhang and C. Liu. 2007. Genetic diversity of wheat gene pool of recurrent selection assessed by microsatellite markers and morphological traits. Euphytica, 155: 249-258. [DOI:10.1007/s10681-006-9326-x]
15. Maccaferri, M., M.C. Sanguineti, P. Donini and R. Tuberosa. 2003. Microsatellite analysis reveals a progressive widening of the genetic basis in the elite durum wheat germplasm. Theoretical and Applied Genetics, 107(5): 783-797. [DOI:10.1007/s00122-003-1319-8]
16. Manifesto, M.M., A.R. Schlatter, H.E. Hopp, E.Y. Suárez and J. Dubcovsky. 2001. Quantitative evaluation of genetic diversity in wheat germplasm using molecular markers. Crop Science, 41(3): 682-690. [DOI:10.2135/cropsci2001.413682x]
17. Maroof, M.A.S., R.M. Biyashev, G.P. Yang, Q. Zhang and R.W. Allard. 1994. Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proceedings of the National Academy of Sciences, 91(12): 5466-5470. [DOI:10.1073/pnas.91.12.5466]
18. Mohammadi, M., R.G. Mirfakhraii and A. Abbasi. 2013. Genetic diversity in bread wheat (Triticum aestivum L.) as revealed by microsatellite markers and association analysis of physiological traits related to spring cold stress. Genetic Novin, 3: 279-288 (In Persian).
19. Mohammadi, S.A. and B.M. Prasanna. 2003. Analysis of genetic diversity in crop plants salient statistical tools and considerations. Crop Science, 43(4): 1235-1248. [DOI:10.2135/cropsci2003.1235]
20. Perry, D.J. 2004. Identification of Canadian durum wheat varieties using a single PCR. Theoretical and Applied Genetics, 109(1): 55-61. [DOI:10.1007/s00122-004-1597-9]
21. Plaschke, J., M.W. Ganal and M.S. Röder. 1995. Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theoretical and Applied Genetics, 91: 1001-1007. [DOI:10.1007/BF00223912]
22. Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and A. Rafalski. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2(3): 225-238. [DOI:10.1007/BF00564200]
23. Prasad, M., R.K. Varshney, J.K. Roy, H.S. Balyan and P.K. Gupta. 2000. The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theoretical and Applied Genetic, 100(3-4): 584-592. [DOI:10.1007/s001229900102]
24. Röder, M.S., V. Korzun, K. Wendehake, J. Plaschke, M.H. Tixier, P. Leroy and M.W. Ganal. 1998. A microsatellite map of wheat. Genetics, 149(4): 2007-2023.
25. Röder, M.S., J. Plaschke, S.U. König, A. Börner, M.E. Sorrells, S.D. Tanksley and M.W. Ganal. 1995. Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics, 246(3): 327-333. [DOI:10.1007/BF00288605]
26. Saremi Rad, B., M. Shokrpor, O. Sofalian, A. Pormuhammad and E. Esfandiari. 2016. Evaluation of genetic diversity of wheat genotypes by AFLP markers. Journal of Crop Breeding, 7(16): 89-96 (In Persian).
27. Wei, L., C. Bian, Y. Wei, A. Liu, G. Chen, Z. Pu and Y. Zheng. 2013. Evaluation of genetic diversity of Sichuan common wheat landraces in China by SSR markers. Journal of Integrative Agriculture, 12(9): 1501-1511. [DOI:10.1016/S2095-3119(13)60418-9]
28. Wu, K.S. and S.D. Tanksley. 1993. Abundance, polymorphism and genetic mapping of microsatellites in rice. Molecular Genetics and Genomics, 241: 225-235. [DOI:10.1007/BF00280220]
29. Zargani, M., G.A. Ranjbar and Sh. Ebrahim Nejad. 2015. Molecular assessment of genetic diversity among bread wheat (Triticum aestivum L.) doubled haploid lines using SSR markers. Journal of Crop Breeding, 7(15): 88-95 (In Persian).

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