Journal of Crop Breeding

  Molecular markers are considered as useful tools for breeding , screening and genetic characterization of genotypes . In crop plants, using of marker assisted selection (MAS) for traits such as product quality and resistance to pests and diseases is being widely adopted by numerous breeding programs worldwide. In current study effects of high molecular glutenin subunit loci Glu1, a lleles 2*, 17+18 and 5+10 were shown to be the most effective alleles for increasing gluten quality, respectively. In present study, we have screened F₂ progenies of a composite cross (M-83-17/3/Dove”S”/Buc”S”//2*Darab/4/Pishtaz/3/Ww33/Vee”S”//Niknejad) belonging to breeding program for temperate climate regions of Iran for presence of 2*, 17+18 and 5+10 alleles. Forty individuals from F₂ generation were tagged in the field. Sequence tagged site (STS) primers Were used as specific markers to test for the presence of 2*, 17 +18 and 5+10 in progenies. Our genotyping tests have revealed that out of 40 progenies, 12 individuals possessed the three alleles. Six out of 12 F₃ progenies with 10 samples were selected and have been transferred to F₄ generation. Results obtained from molecular markers experiment were in agreement with the results from SDS sedimentation test. It is indicated that 2*, 17+18 and 5+10 alleles have positive correlation with SDS-sedimentation volume. Cluster analysis based on between-group variances recognized five different clusters for high quality alleles with different combinations of the three alleles mentioned and three clusters for SDS-sedimentation volume with high, medium and low SDS-sedimentation volume, respectively.