Study on salt tolerance of 35 lines derived gamma ray irradiation on 032 soybean cultivar (M2 generation) were conducted. Seeds of selected M1 lines were sown in greenhouse with 3 levels of sodium chloride (30, 60 and 90 mM) in sandy culture in factorial experiment on the basis of randomized complete block design with 3 replications in 2008. After 60 days salinity treatment, the traits of stem length, root length, root node number, plant fresh weight, dry weight of aerial part of plant, root dry weight and plant biomass were recorded. Analysis of variance indicated that there were significant differences between salinity, lines and interaction between salinity and lines. Also results showed significant differences in morphological traits. The observations showed the most extensive variation range was belong to 320 Gy dose of gamma ray. In this study, 10 lines were selected and they were tolerant to all salinity levels. These promising lines can be used as new germplasms to obtain new salt tolerance varieties in near future.

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