Volume 9, Issue 24 (3-2018)                   J Crop Breed 2018, 9(24): 87-94 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rahmati H, Farshadfar M, Shirvani H. (2018). Study of Genetic Diversity of Festuca Arundinacea Based on ISSR Molecular Markers. J Crop Breed. 9(24), 87-94. doi:10.29252/jcb.9.24.87
URL: http://jcb.sanru.ac.ir/article-1-940-en.html
1- Department of Agriculture Payame Noor University
Abstract:   (4860 Views)
Fescue is an important forage species growing in wide climates. Genetic variation was estimated for 18 accessions of Festuca arundinacea using 12 ISSR primers in payame Noor university of Kermanshah. In total 87 bands (loci) were obtained.77 bands out of 87 were polymorph in accessions. The mean polymorphism percentage was 89.05 among Fescue accessions.The greatest polymorphism percentage were belonged to IS6, IS9, IS11, IS12 and IS16. Polymorphic information content (PIC) average and Marker index (MI) mean were 0.33 and 2.2 respectively. The highest MI belonged to IS10, IS2, IS6 and IS12. The IS10 and IS6 had the most polymorphism. The greatest similarity belonged to accession 16 (kamiaran)-17 (Irland) and the least similarity belonged to accession1 (Bane)-12(Brojen). Cluster analysis and principal coordinate analysis classified these genotyped to four groups; the accessions of the first group had the highest genetic distance with the accessions of the group 3, based on ISSR markers. These results confirmed by molecular variance analysis as well.
 
 
Full-Text [PDF 452 kb]   (1866 Downloads)    
Type of Study: Research | Subject: اصلاح نباتات، بیومتری
Received: 2018/03/10 | Accepted: 2018/03/10

References
1. Brantestam, A.K., R.V. Bothmer, C. Dayteg I. Rashal, S. Tuvesson and J. Weibull. 2004. Inter-simple sequence repeats analysis of genetic diversity and relationship in cultivated barleyof Nordic and Baltic origin. Heredity, 141: 186-192. [DOI:10.1111/j.1601-5223.2004.01867.x]
2. Briggs, D.S. and M. Walters. 1997. Plant variation and evolution. Cambridge University Press, UK, 512 p.
3. Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem bull, 19: 11-15.
4. Fasih, Z., M. Farshadfar and H. Safari. 2013. Genetic diversity evaluation of within and between populations for Festuca arundinacea by ISSR markers. International Journal of Agriculture and Crop Sciences, 5: 135-139.
5. Fortune, J.A. 1989. Forage Plant Breeding. Analysis variation in sainfoin (Onobrychis. Viciofolia). School of Agriculture Science Group, 305-306.
6. Henrry, R.I. 1997. Practical applications of plant molecular biology. Chapman and Hall, London. 258p.
7. Hou, Y., Z. Yan and Y. Wei. 2005. Genetic diversity in barely from west China based on RAPD and ISSR analysis Barely. Genetics Newsletter, 35: 9-22.
8. Khayam Nekoie, M., R. Jahantighi, M. Soloki, R. Muhamadi and A.B. Iman Jome. 2010. Study of genetic variation in different Festuca arundinacea Schreb Using molecular marker AFLP. Journal of Agricultural Sciences and Natural Resources, 1: 351-361.
9. Mahjoob, B., H. Najafi-Zarini and S.HR. Hashemi. 2014. Assessment of Genetic Relationships among 36 Brassica Genotypes using ISSR Molecular Markers. Journal of Crop Breeding, 6: 96-106.
10. Mehdikhani, H., S. Mahmood and H. Zeinali. 2013. Study of Genetic Diversity in Chamomile Landraces (Matricaria aurea (Loefl.) Sch. Bip.) Using Random and Semi-Random Primers. Journal of Crop Breeding, 5: 69-82.
11. Michaud. R., G. Tremblay, F. Belanger and G. Michaud. 1990. J. Agriculture and Agri-Food Canada, Soils and Crops Research and Development Center, 2560 Hochelaga blvd, Sainte-Foy, Quebec, Canada, 213 pp.
12. Peter-schmid, M., R. Kolliker and B. Boller. 2010. Genetic Diversity of Festuca Pratensis Huds and Lolium Multiflorum Lam Ecotype Populations in Relation to Species Diversity and Grassland Type. In: Runas, J. and Dahlgren, Grassland Biodiversity-Habitat Types, Ecological.Nova Science Publishers, Portland, USA, pp: 333-345.
13. Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and A. Rafalski. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238. [DOI:10.1007/BF00564200]
14. Rouf Mian, M.A., A.A. Hopkins and J.C. Zwonitzer. 2002. Determination of Genetic Diversity in Tall Fescue with AFLP Markers. Crop Science, 42: 944-950. [DOI:10.2135/cropsci2002.9440]
15. Saedi, H., M. Shama and K. Nikpour Tehrani. 2001. Tehran University, 337 pp.
16. Sharifi-Tehrani, M., M. Mardi, J. Sahebi, P. Catalán and A. Díaz-Pérez. 2009. Genetic diversity and structure among Iranian tall fescue populations based on genomic-SSR and EST-SSR marker analysis. Plant Systematics and Evolution, 282: 57-70. [DOI:10.1007/s00606-009-0207-3]
17. Souframanien, J. and T. Gopalkrishna. 2004. A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theoretical and Applied Genetics, 109: 87-93. [DOI:10.1007/s00122-004-1797-3]
18. Surve-Iyer, R.S., G.C. Adams, A.F. Lezzon and A.L. Jones. 1995. Isozyme detection and variation in Ieucostoma species from prunus and malus. Mycologia, 87: 471- 482. [DOI:10.1080/00275514.1995.12026557]

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | Journal of Crop Breeding

Designed & Developed by: Yektaweb