Volume 11, Issue 29 (3-2019)
jcb 2019, 11(29): 161-168 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
mahmoudi A, danesh M. (2019). Assessment of Salinity Effects on Some Morphological and Physiological Traits and In Vitro Culture of Halophyte Plant (Salicornia Europaea) . jcb. 11(29), 161-168. doi:10.29252/jcb.11.29.161
URL: http://jcb.sanru.ac.ir/article-1-823-en.html
URL: http://jcb.sanru.ac.ir/article-1-823-en.html
Faculty of Agriculture, Guilan University
Abstract: (3080 Views)
- Salicornia europaea is a halophytic annual plant of the Chenopodiaceae family, which is considered as an oil plant in marine irrigation. This experiment was conducted to determine the effect of salinity and to reach the best hormonal treatment for shoot regeneration. After washing and sterilization, seeds of S. europaea were cultured under controlled light and temperature conditions at five salinity levels consisting of 0, 100, 200, 400 and 600 mM of NaCl in plots including sterilized soil and vermicompost for 30 days. Germination percentage, germination rate, plant height, the fresh and dry weight of the plant, and relative water content were then recorded. For in vitro culture, the shoot-tips were excided and cultivated in Murashige and Skoog medium containing combinations of various plant growth regulators (BA and NAA including 0 and 100 mM salinity levels) under laboratory conditions, and then plant regeneration was investigated. These experiments were performed in a completely randomized design (CRD) with three replicates. The results showed that high salinity exerted a low effect on seedlings of the Salicornia and the rest of traits indicated high levels of salinity tolerance and showed plant high ability to adapt to salinity. In tissue culture experiment, the results of shoot induction indicated that the hormonal treatments in combination with 100 mM of NaCl provided the better regeneration than that of non-salinity conditions. Increasing use of the hormone were not given the better regeneration results, but it was needed to use a good proportion of hormone level for shoot regeneration. In the present research, the best possible ratio was the combination of 0.5 mg/l-1 NAA + 0.5 mg/l BA + 0.5 + 100mM NaCl, which resulted in 66.67 regeneration percentage.
Type of Study: Research |
Subject:
بيوتكنولوژي گياهي
Received: 2017/09/20 | Revised: 2019/05/14 | Accepted: 2018/06/2 | Published: 2019/05/8
Received: 2017/09/20 | Revised: 2019/05/14 | Accepted: 2018/06/2 | Published: 2019/05/8
References
1. Aghaleh, M., V.A. Niknam, H.A. Ebrahimzadeh and K.B. Azavi. 2009. Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. europaea. Biologia Plantarum, 53(2): 243-248. [DOI:10.1007/s10535-009-0046-7]
2. Aghaleh, M., V.A. Niknam, H.A. Ebrahimzadeh and K.B. Razavi. 2011. Effect of salt stress on physiological and antioxidative responses in two species of Salicornia S. persica and S. europaea. Acta Physiologiae Plantarum, 33(4): 1261-1270. [DOI:10.1007/s11738-010-0656-x]
3. Amiri, B., M.H. Assareh, M. Jafari, B. Rasuoli, H. Arzani and A.A. Jafari. 2010. Effect of salinity on growth, ion content and water status of glasswort (Salicornia herbacea L.). Caspian Journal of Environmental Sciences, 8(1): 79-87.
4. Ayala, F. and J.W. O'Leary. 1995. Growth and physiology of Salicorni bigelovii Torr. At suboptimal salinity .International Journal of Plant Sciences, 156(2): 197-205. [DOI:10.1086/297241]
5. Bond, J.E. and M.L. Roose. 1998. Agrobacterium-mediated transformation of the commercially important citrus cultivar Washington navel orange. Plant Cell Reports, 18(3-4): 229-234. [DOI:10.1007/s002990050562]
6. Ghasemi Bezdi, K. and B. Aidin. 2012. Study of callus formation and salinity tolerance in different explants of Cotton (Gossypium sp.) cultivars in vitro. Journal of Crop Breeding, 4(102): 94-108 (In Persian).
7. Ghosh, P.K., M.P. Reddy, J.B. Pandya, J.S. Patolia, S.M. Vaghela, M.R. Gandhi, R.J. Sanghvi V.G.S. Kumar and M.T. Shah. 2005. Preparation of nutrient rich salt of plant origin. US patent No, 6: 929-809.
8. Glenn, E., W.O. Leary and W. Corolyn. 1991. Salicornia bigelovii Torr: an oilseed halophyte for seawater irrigation. Science, 251: 1065-1067. [DOI:10.1126/science.251.4997.1065]
9. Gul, B. and D.J. Weber. 1999. Effect of salinity, light, and thermo period on the seed germination of Allenrolfea occidentalis. Canadian Journal of Botany, 77(2): 240-246. [DOI:10.1139/b98-204]
10. Gregorio, B.G., D. Senadhira and R.D. Mendza. 1997. Screening rice for salinity tolerance. Manuals of plant breeding, Genetics and Biochemistry division, IRRI discussion paper series, 22: 1-30.
11. Grigoriadou, K. and E. Maloupa. 2008. Micro propagation and salt tolerance of invitro grown Crithmum maritimum L. Plant Cell Tissue Organ Culture, 94: 209-217. [DOI:10.1007/s11240-008-9406-9]
12. Hogan, W.C. 1968. The effect of salinity on the germination and the growth of two halophytes. M.S. Thesis, Ohio University, 38 pp.
13. Joshi, M., A. Mishra and B. Jha. 2012. NaCl plays a key role for in vitro micro propagation of Salicornia brachiata, an extreme halophyte. Industrial Crops and Products, 35: 313-316. [DOI:10.1016/j.indcrop.2011.06.024]
14. Joshi, M., A. Mishra and B. Jha. 2011. Efficient genetic transformation of Jatropha curcas L. by microprojectile bombardment using embryo axes. Industrial Crops and Products journal, 33(1): 67-77. [DOI:10.1016/j.indcrop.2010.09.002]
15. Katschnig, D., R. Broekman and J. Rozema. 2012. Salt tolerance in the halophyte Salicornia dolichostachya Moss: growth, morphology and physiology. Environmental and Experimental Botany, 92: 32-42. [DOI:10.1016/j.envexpbot.2012.04.002]
16. Kadereit, G., P. Ball, S. Beer, L. Mucina, D. Sokoloff, P.Teege1, A.K. Yaprak and H. Freitag. 2007. A taxonomic nightmare comes true: phylogeny and biogeography of glassworts (Salicornia L., Chenopodiaceae). International Bureau for Plant Taxonomy and Nomenclature, 56(4): 1143-1170. [DOI:10.2307/25065909]
17. Keiffer, C.W. and I.A. Ungar. 1995. Germination responses of halophyte seeds exposed to prolonged hypersaline conditions. In: Khan, M.A. and Ungar, I.A. (Eds) Biology of Salt Tolerant Plants. pp., Department of Botany, University of Karachi, Pakistan, 43-50.
18. Keller, W.A., R. Rajhathy and J. Lacapra. 1975. In vitro production of plants from pollen in Brassica campestris. Canadian Journal of Genetics and Cytology, 17(4): 655-666. [DOI:10.1139/g75-081]
19. Khan, M.A., B. Gul and D.J. Weber. 2001. Effect of salinity on the growth and ion content of Salicornia rubra. Communications in Soil Science and Plant Analysis, 32: 2965-2977. [DOI:10.1081/CSS-120000975]
20. Khan, M.A. and I.A. Ungar. 1996. Influence of salinity and temperature on the germination of Haloxylon recurvum. Annals of Botany, 78(5): 547-551. [DOI:10.1006/anbo.1996.0159]
21. Khan, M.A. and I.A. Ungar. 1997. Germination responses of the subtropical annual halophyte Zygophyllum simplex. Seed Science and Technology, 25: 83-91.
22. Khan, M.A. and B. Gul. 1998. High salt tolerance in germinating dimorphic seeds of Arthrocnemum indicum. International Journal of Plant Science, 159(5): 826-832. [DOI:10.1086/297603]
23. Khan, M.A. and I.A. Ungar. 1998. Germination of salt tolerant shrub Suaeda fruticosa from Pakistan: Salinity and temperature responses. Seed Science and Technology, 26: 657-667.
24. Khan, M.A., A.I. Unger and A.M. Showalter. 2000. The effect of salinity on the growth, water status, and ion content of a leaf succulent perennial halophyte, Suaeda fruticosa (L) Forssk. Journal of Arid Environments, 45(1): 73-84. [DOI:10.1006/jare.1999.0617]
25. Khan, M. and D. Weber. 1986. Factors influencing seed germination in Salicornia pacifica var. utahensis. American Journal Botany, 73(8): 1163-1167. [DOI:10.1002/j.1537-2197.1986.tb08562.x]
26. Kong, Y. and Z. Youbin. 2014. Potential of Producing Salicornia bigelovii hydroponically as a Vegetableat Moderate NaCl Salinity. American Society for Horticultural Science, 49(9):1154-1157. [DOI:10.21273/HORTSCI.49.9.1154]
27. Lee, C.W., E.P. Glenn and J.W. O'Leary. 1992. In vitro propagation of Salicornia bigelovii by shoot-tip cultures. HortScience, 27(5): 472. [DOI:10.21273/HORTSCI.27.5.472]
28. Li, Y., Y. Zhang, F. Feng, D. Liang, L. Cheng, F. Ma and S. Shi. 2010. Overexpression of a Malus vacuolar Na+/H+antiporter gene (MdNHX1) in apple rootstock M.26 and its influence on salt tolerance. Plant Cell Tissue Organ Culture, 102(3): 337-345. [DOI:10.1007/s11240-010-9738-0]
29. Loveland, D. and L. Ungar. 1983. The effect of nitrogen fertilization on the production of halophytes in an inland salt marsh. American Midland Naturalist, 109(3): 346-354. [DOI:10.2307/2425415]
30. Munns, R., R.A. James and A. Läuchli. 2006. Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany, 57(5): 1025-1043. [DOI:10.1093/jxb/erj100]
31. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-479. [DOI:10.1111/j.1399-3054.1962.tb08052.x]
32. O'Leary, J.W., E.P Glenn, M.C. Watson. 1985. Agricultural production of halophytes irrigated with seawater. Plant Soil, 89(1-3): 311-321. [DOI:10.1007/BF02182250]
33. Parks, G.E., M.A. Dietrich and K.S. Schumaker. 2002. Increased vacuolar Na+/H+ exchange activity in Salicornia bigelovii Torr. In response to NaCl. Journal Experimental Botany, 53(371): 1055-1065. [DOI:10.1093/jexbot/53.371.1055]
34. Park, K.W., J.Y. An, H.J. Lee, D. Son, Y.G. Sohn, C. Kim and J.J. Lee. 2013. The growth and accumulation of osmotic solutes of the halophyte common glasswort (Salicornia europaea) under salinity conditions. Journal of Aquatic Plant Management, 51: 103-108.
35. Rozema, J. and H. Schat. 2013. Salt tolerance of halophytes, research questions reviewed in the perspective of saline agriculture. Environmental and Experimental Botany, 92: 83-95. [DOI:10.1016/j.envexpbot.2012.08.004]
36. Shabala, S. and A. Mackay. 2011. Ion transport in halophytes, In: Turkan, S. (eds.) plant responses to drought and salinity stress Developments in a Post-Genomic Era. Academic Press Ltd-Elsevier Science Ltd, London, 151-199 pp. [DOI:10.1016/B978-0-12-387692-8.00005-9]
37. Schonfield, M.P., J.C. Richard, B.P. Carver and N.W. Mornhi. 1988. Water relations in winter wheat as drought resistance indicators, Crop Science, 28(3): 526-531. [DOI:10.2135/cropsci1988.0011183X002800030021x]
38. Shi, X.L., H.P. Han, W.L. Shi and Y.X. Li. 2006. NaCl and TDZ are two key factors for the improvement of in vitro regeneration rate of Salicornia europaea L. Journal of Integrative Plant Biology, 48(10): 1185-1189. [DOI:10.1111/j.1744-7909.2006.00342.x]
39. Shahin Kaleybar, B., G. Nematzadeh, S.H.R. Hashemi, H. Askari and S. Kabirnataj. 2013. Physiological and Genetic Responses of Halophyte Aeluropus littoralis to Salinity. Journal of Crop Breeding, 5(12): 15-29 (In Persian).
40. Soltani, A., S. Galeshi, F. Zenali and N. Latifi. 2001. Germination seed reserve utilization and growth of Chicpea as affected by salinity and seed size. Seed Sci and Technol, 30(1): 51-40.
41. Ungar, I.A. 1962. Influence of salinity on seed germination in succulent halophytes. Ecology, 3(4): 329-335. [DOI:10.2307/1933476]
42. Uno, Y., S. Nakao, Y. Yamai, R. Koyama, M. Kanechi and N. Inagaki. 2009. Callus formation, plant regeneration, and transient expression in the halophyte sea aster (Aster tripolium L.). Plant Cell Tissue Organ Culture, 98(3): 303-309. [DOI:10.1007/s11240-009-9564-4]
43. Volaire, F., H. Thomas and F. Leli'evre. 1998. Survival and recovery of perennial forage grasses under prolonged Mediterranean drought: I. Growth, death, water relations and solute content in herbage and stubble. The New Phytologist, 140(3): 439-449. [DOI:10.1046/j.1469-8137.1998.00288.x]
44. Watkins, C.B., J.M. Brown and F.J. Dromegoole. 1988. Salt-tolerance of the coastal plant, Tetragonia trigyna Banks ET Sol. ex Hook. (Climbing New Zealand spinach). New Zealand journal of botany, 26(1): 153-162. [DOI:10.1080/0028825X.1988.10410107]
45. Yoshie, S.M. and K. Hideo. 1994. Studies on the mechanism of salt tolerance in Salicornia europaea. Japanese Journal of Crop Science, 63(3): 518-523. [DOI:10.1626/jcs.63.518]
46. Zhao, K.F. and L.T. Feng. 2001. Halophyte Resources in China. Science Press, Beijing, 491-498 pp.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
