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Abstract:   (44 Views)
The increasing trend of extinction of valuable plant species such as Thymus daenensis Celak due to various biological and non-biological factors requires an overview of conservation and preservation. In this regard, cryopreservation techniques are one of the most important methods for keeping seed in non-habitat conditions. In the present study, the effect of different plant vitrification solutions (PVS2 and PVS3) using two methods of vitrification (encapsulation-vitrification and droplet-vitrification) on the longitudinal and germination indices of Thymus daenensis seeds were evaluated. The results showed that the highest viability rate (98%) and seed vigor index (26) was obtained after droplet-vitrification method using PVS3 (P ≤0.05). Also, the lowest seedlings height was observed in encapsulation-vitrification method using PVS2 (17/33 mm; P ≤0.05). Therefore, droplet-vitrification method using PVS3 is more favorable than other methods for the long-term preservation of Thymus daenensis seeds.
 
     
Type of Study: Research | Subject: بيوتكنولوژي گياهي

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