Volume 10, Issue 28 (12-2018)                   jcb 2018, 10(28): 155-161 | Back to browse issues page


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abdolahnezhad E, poozesh V, zavareh S. Comparison of the Different Plant Germplast Cryopreservation Methods on Growth and Germination Indices of Thyme (Thymus Daenensis). jcb. 2018; 10 (28) :155-161
URL: http://jcb.sanru.ac.ir/article-1-809-en.html
Faculty of Biology and Research Institute of Life Sciences, Damghan University
Abstract:   (933 Views)
Increasing rate of extinction of valuable plant species such as Thymus daenensis Celak due to various biological and non-biological factors requires an overview of conservation and preservation methods. In this regard, cryopreservation techniques are one of the most important methods for preservation of seeds in non-habitat conditions. In the present study, the effects of different plant vitrification solutions (PVS2 and PVS3) were evaluated using two methods of vitrification (encapsulation-vitrification and droplet-vitrification) on the longitudinal and germination indices of T. daenensis seeds. Results showed that the highest viability rate (98%) and seed vigor index (26) was obtained after droplet-vitrification method using PVS3 (p ≤0.05). Also, the lowest seedlings height was observed in encapsulation-vitrification method using PVS2 (17/33 mm; p ≤0.05). Therefore, droplet-vitrification method using PVS3 is more favorable for the long-term preservation of T. daenensis seeds.
 
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Type of Study: Research | Subject: بيوتكنولوژي گياهي
Received: 2017/08/1 | Revised: 2019/03/2 | Accepted: 2018/05/30 | Published: 2019/03/2

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