Volume 10, Issue 28 (12-2018)
J Crop Breed 2018, 10(28): 38-49 |
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parand M, yamchi A, Soltanloo H, Zaynalinejad K. (2018). Study of Morphological Traits and Genetic Diversity of low Molecular Wight-Glutenin Subunits in Some Bread Wheat Cultivars using SRAP Markers. J Crop Breed. 10(28), 38-49. doi:10.29252/jcb.10.28.38
URL: http://jcb.sanru.ac.ir/article-1-754-en.html
URL: http://jcb.sanru.ac.ir/article-1-754-en.html
Abstract: (3924 Views)
Baking quality is one of the most important traits in wheat quality breeding. In the present study, allelic diversity of the genes encoding glutenin with low molecular weight (LMW-GS) was evaluated in 15 good, average and poor cultivars in term of baking quality using SRAP markers. Further, morphological traits, including 100-seed weight, spike number per plot, seed number per spike, plant height and spike length were investigated in order to identify possible correlation with molecular markers. In this experiment, two SRAP markers were designed based on repetitive region in LMW-GS. The ANOVA results of morphological traits revealed that all cultivars were differently at 0.01level. Additionally, the correlation analysis between grain yield and other morphological traits indicated a high correlation between yield and spike number per plot. In morphological traits, the cultivars were grouped in three cluster using WARD method and CCC plot cutoff. The product size ranged from 200 to 3000 bp and 90 to 2500 bp for SRAP1 and SRAP2 markers, respectively. In total, 19 bands were produced among the cultivars and polymorphic percentage was 42.1. SRAP2 produced the highest number of bands (11). Polymorphic information content (PIC) was 0.11 and 0.39 for SRAP1 and SRAP2 markers, respectively. Cluster analysis based on Jaccard’s coefficient and UPGMA algorithm by NTSYS-pc 2.2 software related that the cultivars were allocated in four clusters. The results showed that SRAP marker could approximately group the cultivars according to baking trait and this classification can be compared with morphological data.
References
1. Aghaee-Sarbaze, M. 2012. Agronomic Traits in Durum wheat variety. Journal of Seed and Plant Improvement, 1(3): 481-502. (In Persian)
2. Alvin, C. R. and F. C. William. 1995. Methods of multivariate analysis. Wiley press. 800 w.
3. Arzani, A. 1999. Breeding of crop plants. Isfahan University Press. 320 w.
4. Babae Zarrch, M., M. Fatookian and S. Mahmudi. 2013. Assessment of genetic diversity morphological traits wheat using multivariate. Journal of plant breeding agricultural, 5(1). (In Persian)
5. Bahrae, C. 2003. Iranian bread wheat quality assessment based on glutenin subunits heavy. Journal of Crop Science, 5: 3-17. (In Persian)
6. Bozorgmehr, A., J. Ahmadi, F. Shahinnia, Kh. Razavi, G. Njafian and T. Lohrasebi. 2014. Evaluation of allelic variation for low molecular weight glutenin subunits using DNA specific markers in wheat landraces. Journal of Modern Genetics, 9(4): 439-450. (In Persian)
7. Cassidy, B.G., J. Dvorak and O.D. Anderson. 1998. The wheat low molecular weight glutenin genes: characterization of six new genes and progress in understanding gene family structure. Theoretical and Applied Genetics, 96: 743-750. [DOI:10.1007/s001220050797]
8. Collaku, A. and S.A. Harrison. 2002. Losses in wheat due to water logging. Crop Science, 42: 444-450. [DOI:10.2135/cropsci2002.0444]
9. D'Ovidio, R., C. Marchitelli, L. Ercoli Cardelli and E. Porceddu. 1999. Sequence similarity between allelic Glu-B3 genes related to quality properties of durum wheat. Theoretical and Applied Genetics, 98, 455-461. [DOI:10.1007/s001220051091]
10. D'ovidio, R. and S.M. Masci. 2004. The low-molecular-weight glutenin subunits of wheat gluten. Journal of Cereal Science, 39(1), 321-339. [DOI:10.1016/j.jcs.2003.12.002]
11. Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19:11-15.
12. Farahani,A. and A. Arzani. 2006. The study of genetic diversity among durum wheat and F1 generation using agronomic traits. Journal of Agriculture, 4: 341-354. (In Persian)
13. Fernando, R., R. Cuillenportal, N. Obert, S. Qingwnxueaudkent and M. Eskridge .2006. Compensatory mechanisms associated with the effect of spring wheat seed size and wild oat competition. Crop Science, 46: 935-945. [DOI:10.2135/cropsci2005.08-0270]
14. Gill, B.S., R. Appels, A.M. Botha-Oberholster, C.R. Buell, J.L. Bennetzen, B. Chalhoub and B. Keller. 2004. A workshop report on wheat genome sequencing international genome research on wheat consortium. Genetics, 168(2), 1087-1096. [DOI:10.1534/genetics.104.034769]
15. Halajian, M. and B. Naserian. 2007. Review and compare amino acid sequences x and y types glutenin subunits loci 1 D controller bread quality wheat. 12th Iranian Biotechnology Conference, Tehran.
16. Harberd, N.P., D. Bartels and R.D. Thompson. 1985. Analysis of the gliadin multigene loci in bread wheat using nullisomic-tetrasomic lines. Molecular and General Genetics, 198, 234-242. [DOI:10.1007/BF00383001]
17. Iran-Nejad, H., and N. Shahbaziyan. 2005. Cereal cultivation. Wheat. Karenoo Publications.Tehran, Iran, 272 p. (In Persian)
18. Jackson, E.A., L.M. Holt and P.I. Payne. 1983. Characterisation of high molecular weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal localisation of their controlling genes. Theoretical and Applied Genetics, 66: 29-37. [DOI:10.1007/BF00281844]
19. Johansson, E., G. Svensson and W. K. Heneen. 1998. Genotype and environmental effect on factors influencing bread-making quality. In: A. E. Slinkard ed. Proc. 9th Intl. Wheat Genetics Symp 4: 175-177.
20. Lee, M. 1995. DNA markers and plant breeding programs. Advances in Agronomy, 55:265-344. [DOI:10.1016/S0065-2113(08)60542-8]
21. Li, G. and C.F. Quiros. 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 103: 455-461. [DOI:10.1007/s001220100570]
22. Li, W., Z. Gao,Y. M. Wei, Z. E. Pu, G. Y. Chen, Y. X. Liu, H. P. Chen, X. J. Lan and Y. L. Zheng. 2012. Genetic variations of m-type LMW-GS genes and their associations with dough quality in Triticum turgidum ssp. Turgidum landraces from China. African Journal of Agricultural Research, 7: 2025-2033. [DOI:10.5897/AJAR11.2310]
23. Lin X., S. Kaul, S. Rounsley, T. Shea and M.I. Benito. 1999. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana. Nature, 402:761-767. [DOI:10.1038/45471]
24. Martinek, P., M. Vinterova, I. Burešová and T. Vyhnanek. 2008. Agronomic and quality characteristics of triticale (X Triticosecale Wittmack) with HMW glutenin subunits 5+10. Journal of Cereal Science, 47(1): 68-78. [DOI:10.1016/j.jcs.2007.02.003]
25. Masci, S., D. Lafiandra, E. Porceddu, E.J.L. Lew, H.P. Tao, D.D. Kasarda. 1993. D-glutenin subunits: N-terminal sequences and evidence for the presence of cysteine. Cereal Chemistry, 70: 581-585.
26. Masci, S., E.J.L. Lew, D. Lafiandra, E. Porceddu and D.D. Kasarda. 1995. Characterization of low-molecular-weight glutenin subunits in durum wheat by RP-HPLC and N-terminal sequencing. Cereal Chemistry, 72: 100-104.
27. Masci, S., L. Rovelli, D.D. Kasarda, W.H. Vensel and D. Lafiandra. 2002. Characterisation and chromosomal localization of C-type low molecular- weight glutenin subunits in the bread wheat cultivar Chinese Spring. Theoretical and Applied Genetics 104: 422-428. [DOI:10.1007/s001220100761]
28. Mirakhoondi, N. 2001. Study of Variation of Quantitative Traits and Their relationships with yield under drought conditions and irrigation and The best indicator of drought tolerance in durum wheat. Master's Thesis. School of Agriculture. University of Tehran. Karaj, Iran. (In Persian)
29. Mousavi shabestari, M. 2007. Check wheat yield in 21 cold areas. Master's Thesis. Islamic Azad University, Tabriz, Iran. (In Persian)
30. Nei, M. 1973. Analysis of gene diversity in subdivided populations Proceedings of the National Academy of Sciences of the United States of America, 70: 3321-3323. [DOI:10.1073/pnas.70.12.3321]
31. Pahlevani, S., A., Izanloo, S., Parsa and M.GH. Ghaderi. 2016. Association between grain quality traits and SSR molecular markers in some bread wheat genotypes. Journal of Crop Breeding. 8 (19): 25-36. (In Persian)
32. Payne, P.I., Jackson, E.A., Holt, L.M., 1984. The association between ggliadin 45 and gluten strength in durum wheat varieties. A direct causal effect on the result of genetic linkage. Journal of Cereal Science 2, 73-81. [DOI:10.1016/S0733-5210(84)80020-X]
33. Payne, P.I., L.M. Holt, M.G Jarvis and E.A. Jackson. 1985. Twodimensional fractionation of the endosperm proteins of bread wheat (Triticum aestivum): biochemical and genetic studies. Cereal Chemistry, 62: 319-326.
34. Quiros CF., F. Grellet, J. Sadowsk, T. Suzuki, G. Li and T. Wroblewski. 2001. Arabidopsis and Brassica comparative genomics: sequence, structure and gene content in the ABI1-Rps2-Ck1 chromosomal segment and related regions. Genetics. (in press)
35. Rafalski JA., J.M. Vogel, M. Morgante, W. Powell, C. Andre and S. Tingey. 1996. Non-mammalian genome analysis: a practical guide. Academic Press, New York, pp 75-134. [DOI:10.1016/B978-012101285-4/50005-9]
36. Rashidi, V., A. Majidi, V. Mohammadisa and M. Moghadam Vahed. 2007. Determine the genetic relationships of durum wheat lines by cluster analysis and identify morphological traits each group. Journal of Iran Agricultural Sciences, 13(2): 439-450. (In Persian)
37. Sabelli, P. and P.R. Shewry. 1991. Characterization and organization of gene families at the Gli-1 loci of bread and durum wheat. Theoretical and Applied Genetics, 83: 428-434. [DOI:10.1007/BF00226253]
38. Sadeghi, F. and H. Dehghani. 2016. Study of correlation coefficients and factors analysis of bread making quality attributes in bread wheat. Journal of Crop Breeding. 8 (19): 1-8. (In Persian)
39. Seed and Plant Improvement Institute. 2015. Introduced cultivars food security and health, volume 1. Agricultural Extension and Education Research Organization. (In Persian)
40. Shafaedin, S. and B. Yazdi Samadi. 1994. Genetic diversity and geographic wheat mills native to Central Iran. Journal of Iran Agricultural Sciences, 25: 61-77. (In Persian)
41. Shahid Masood, M., A. Javaid, M. Ashiq Rabbani and R. Anwar. 2005. Phenotypic diversity and trait association in bread wheat (Triticum aestivum L.) Landraces from Baluchistan, Pakistan. Pakistan Journal of Botany, 37(4): 949- 957.
42. Shariat, F., S. A. Mohammadi, M. Norouzi and M. Valizadeh. 2015. Allelic diversity of low molecular weight glutenin subunit at Glu-A3, Glu-B3 and Glu-D3 loci in Iranian spring bread wheat landraces. Iranian Journal of Crop Sciences, 17(1):74 -87. (In Persian)
43. Shewry, P.R., N.G. Halford and A.S. Tatham. 1989. The high molecular weight subunits of wheat, barley and rye. In: Miflin, B.J., (Ed.), Genetics, Molecular Biology, Chemistry and Role in Wheat Gluten Structure and Functionality, Oxford Survey Plant Molecular and Cellular Biology, vol. 6. University Press, New York, pp. 163-219.
44. Sidwell, R. J., E.L. Smith and R.W. Mcnew. 1975. inheirtance and interrelationships of grain yield and selected yield related traits in a hard red winter wheat cross. Crop Science, 16: 650-65. [DOI:10.2135/cropsci1976.0011183X001600050013x]
45. Sissons, M.J., B. Osborne and S. Sissons. 2006. Application of near infrared reflectance spectroscopy to a durum wheat breeding programme. Journal of Near Infrared Spectroscopy, 14: 17-25. [DOI:10.1255/jnirs.582]
46. Toosi Mojarad, M. and M. Bihamta. 2007. Check wheat grain yield and other traits through by principal component. Journal of Agricultural Science, 2: 1-97. (In Persian)
47. Vaezi, S. 1999. Genetic diversity and geographic diversity index and quantitative local collection of durum wheat mills in Iran. Master's Thesis. School of Agriculture. University of Tehran. Karaj, Iran. (In Persian)
48. Xu H., R.J. Wang, X. Shen, Y.L. Zhao, G.L. Sun, H.X. Zhao and A.G. Guo. 2006. Functional properties of a new low molecular- weight glutenin subunit gene from a bread wheat cultivar. Theoretical and Applied Genetics, 113: 1295-1303. [DOI:10.1007/s00122-006-0383-2]
49. Zhen, S., C. Han, C. Ma, A. Gu, M. Zhang, X. Shen, X. Li and Y.Yan. 2014. Deletion of the low-molecular-weight glutenin subunit allele Glu-A3a of wheat (Triticum aestivum L.) significantly reduces dough strength and breadmaking quality. BMC Plant Biology, 14: 367-384. [DOI:10.1186/s12870-014-0367-3]
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