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Azad university of Arak
Abstract:   (38 Views)

Finding association between molecular markers and agronomic traits provide an excellent tool for indirect selection of a trait of interest in the population. In this study, stepwise regression analysis was used to estimate associations between ISSR and RAPD markers with some agronomic traits in lemon balm ecotypes. The results analysis revealed significant associations between the traits and some of the studied loci. For all the traits more than one informative marker was detected. Totally 90 informative markers including 48 ISSR loci and 42 RAPD loci were identified. The SA-R-10, UBC826-1, UBC812-9, UBC813-10, UBC825-4, OPA-01-15, OPC-04-7 and CS-56-8 markers showed a significant correlation with Essential oil percentage and controlled 99.8% of the phenotypic variation. These markers are considered to be relatively more reliable. Among the RAPD primers, special attention should be drawn to primer SA-R, which had the highest associated fragments with the traits including days to 50% flowering, number of branches per plant, fresh weight and dry weight. Some of these markers were associated with more than one trait that these traits had very close linkage together or possibly were controlled by pleiotropic effects. These primers have been found useful for improved lemon balm.


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