دوره 10، شماره 26 - ( تابستان 1397 )
جلد 10 شماره 26 صفحات 75-65 |
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Zali H, Sofalian O, Zeinalabedini M, hasanloo T, asgharii A, Alizadeh B. (2018). Assessment of Variability and Genetic Structure of Canola cultivars and Lines using SSR Markers Related on Drought Tolerance QTLs. jcb. 10(26), 65-75. doi:10.29252/jcb.10.26.65
URL: http://jcb.sanru.ac.ir/article-1-749-fa.html
URL: http://jcb.sanru.ac.ir/article-1-749-fa.html
زالی حسن، سفالیان امید، زین العابدینی مهرشاد، حسنلو طاهره، اصغری علی، علیزاده بهرام. بررسی تنوع مولکولی و ساختار ژنتیکی ارقام و لاینهای کلزا با استفاده از نشانگرهای SSR مرتبط با QTLهای تحمل به خشکی پژوهشنامه اصلاح گیاهان زراعی 1397; 10 (26) :75-65 10.29252/jcb.10.26.65
سازمان تحقیقات، آموزش و ترویج کشاورزی
چکیده: (3281 مشاهده)
در این تحقیق تنوع ژنتیکی 41 لاین و ژنوتیپ کلزا شامل 19 رقم آزاد گردهافشان و هیبرید، 17 لاین امیدبخش و 5 لاین هاپلوئید مضاعفشده کلزا با استفاده از نشانگرهای ریزماهواره مرتبط با QTLهای تحمل به خشکی مورد بررسی قرار گرفت. 36 نشانگر مورد مطالعه، 166 نوار قابل تشخیص ایجاد کردند و 157 آلل (58/94%) آن چندشکل بودند که نشان دهنده تنوع ژنتیکی قابل ملاحظه ای در میان لاینها و ژنوتیپها بود. میزان محتوای اطلاعات چندشکلی در بین نشانگرها از 046/0 (FITO133) تا 327/0 (BRMS-204) متغیر بود. متوسط میزان شاخص PIC برابر با 212/0 بود. نتایج نشان داد که متوسط شاخص نشانگری، تنوع ژنی نِی، شاخص اطلاعات شانون و تعداد آلل های مؤثر بهترتیب 508/1، 449/0 و 298/0 بود. در ارزیابی تجزیه ساختار، ارقام و لاینها مورد مطالعه به دو زیر جمعیت تقسیم شدند، که زیر جمعیت اول شامل ارقام Talaye، Hyola420 و Hyola401 و لاینهای هاپلوئید مضاعفشده DH1، DH5، DH8، DH9 و DH11 و زیر جمعیت دوم شامل ارقام پاییزه (سایر ارقام و لاینها) بودند. در تجزیه واریانس مولکولی، واریانس درون جمعیتی بیشتر از واریانس بین جمعیتی بود. در مجموع، نشانگری پیدا نشد که بتوان از آن به عنوان نشانگری درجهت انتخاب ارقام متحمل به خشکی درگام اول برنامه های اصلاحی استفاده کرد و این هدف نیاز به تحقیقات بیشتر در مورد شناسایی QTLهای تحمل به خشکی در گیاه کلزا دارد.
نوع مطالعه: كاربردي |
موضوع مقاله:
اصلاح نباتات مولكولي
دریافت: 1396/1/15 | ویرایش نهایی: 1397/7/7 | پذیرش: 1396/5/22 | انتشار: 1397/7/4
دریافت: 1396/1/15 | ویرایش نهایی: 1397/7/7 | پذیرش: 1396/5/22 | انتشار: 1397/7/4
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