Volume 10, Issue 27 (11-2018)                   jcb 2018, 10(27): 57-65 | Back to browse issues page

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Moslemi H, Solouki M, Fakheri B A. QTLs Analysis for Morphologic Traits of Barley under Boron Stress Condition. jcb. 2018; 10 (27) :57-65
URL: http://jcb.sanru.ac.ir/article-1-476-en.html
Zabol University
Abstract:   (376 Views)

     In present study, 72 barley double haploid lines with two parents were studied. The experiment was arranged in a completely randomized block design with two replicates under normal and boron stress in hydroponic condition in 2013.The traits Fresh and dry weight of root and shoot, length of root and shoot, length of the largest leaf and the height of whole plant were measured. Statistical analysis was done for phenotypic surveys such as analysis of variance, mean comparison and correlation between traits. These results showed meaningful difference among the lines in most traits. Maximum correlation was seen between length of root and the height of whole plant (0/934,0/925). QTL analysis was carried out using genetic linkage map derived 327 AFLP molecular marker and using QTL cartographer software with composite interval mapping method. In general, 29 QTL were found for the studied traits (17 QTL for normal condition and 12 QTL for born stress condition, respectively), in which, explained that the phonotypical variance has been vitiated for 10 to 27.56 percent. The highest and the lowest phonotypic variances were belonging to root and shoot length ratio in born stress condition, The LOD ranged between 2/50-4/32. The highest and lowest LOD were attained for the QTLs of root and shoot length ratio and leaf length, respectively. In percent study, only one QTL was placed at similar place and was stable (leaf length, 3H). Therefore, it can be used for marker assisted selection.

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Type of Study: Research | Subject: اصلاح نباتات، بیومتری
Received: 2015/11/9 | Revised: 2018/12/9 | Accepted: 2016/04/9 | Published: 2018/12/8

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