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gonbad kavous university
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Nitrogen is the most important nutrient macro element that has a role in the structure of different protein molecules, enzymes, co-enzymes, nucleic acids and cytochromes. In order to detect the QTL associated with nitrogen-deficiency tolerance at seedling stage, a population of 96 lines derived from Ahlami Tarom and Neda cross were studied in botany and genetic labrotary of Gonbad Kavous University in 2014. For genetic linkage map construction, 30 SSR and 15 ISSR makers were used. Linkage map covered 1411.3 cM of rice genome with an average of 15.34 cM distance between two markers. A total of 37 QTLs was identified for the traits under study. qRV-10 under conditions of nitrogen deficiency explained 11.4% of the phenotypic variations of the root volume. QTLs controlling root number (qRN-3 and qRN-6b) and root weight (qRW-5b and qRW-5c) under normal conditions were detected in the major stress that this is justified QTL accounted for over 20 percent of phenotypic changes. Markers linked to the QTL as markers linked to traits are likely to be used for marker-assisted selection in breeding programs.


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