Volume 15, Issue 47 (10-2023)
J Crop Breed 2023, 15(47): 165-173 |
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Kianipour A, Baghaeifar Z, Darvishnia H, Saeidian S. (2023). A Comparative Study of Total Phenolic, Total Flavonoid Contents and Antioxidant Capacity of Aerial Parts Extracts of Ferulago angulata in Different Developmental Stages. J Crop Breed. 15(47), 165-173. doi:10.61186/jcb.15.47.165
URL: http://jcb.sanru.ac.ir/article-1-1411-en.html
URL: http://jcb.sanru.ac.ir/article-1-1411-en.html
1- Department of Biology, Faculty of Science, Payame Noor University, Tehran, Iran
Abstract: (1209 Views)
Extended Abstract
Introduction and Objective: Recently, there has been an increased interest in identifying natural antioxidant compounds for use in the pharmaceutical and food industries, mainly due to increased unintentional side-effects of synthetic antioxidants. The present study aimed to evaluate the total phenolic and flavonoid contents and antioxidant activity of ethanolic extract from aerial parts of Ferulago angulata in three developmental stages from two natural habitats of Ilam Province.
Materials and Methods: The extracts were prepared from aerial parts of Ferulago angulate plant. The total phenol and flavonoid contents of extracts were measured spectrophotometrically by using Folin-Ciocalteu reagent and aluminium chloride, respectively. The antioxidant capacity was determined by DPPH method. The results of this method were expressed by calculating the 50% inhibitory concentration (IC50) of the extract in different developmental stages.
Results: The results showed that the highest phenolic and flavonoid content is in the flowering development stage of Kabirkouh habitat. The DPPH scavenging activity results of methanolic extracts showed that the lowest IC50 value (the highest antioxidant activity) and the highest IC50 value (the lowest antioxidant activity) of methanolic extracts were 0.71 and 2.06 mg/ml at developmental flowering and pre-flowering stages, respectively.
Conclusion: All extracts showed antioxidant activity, which is more effective in the developmental flowering stage of Kabirkouh habitat. This plant can be considered an available source of natural antioxidants that can be used in the food and pharmaceutical industries. In the study of two habitats and three developmental stages, Kabirkouh habitat and the flowering developmental stage are suggested as the most suitable habitats and developmental time to collect this medicinal plant.
Introduction and Objective: Recently, there has been an increased interest in identifying natural antioxidant compounds for use in the pharmaceutical and food industries, mainly due to increased unintentional side-effects of synthetic antioxidants. The present study aimed to evaluate the total phenolic and flavonoid contents and antioxidant activity of ethanolic extract from aerial parts of Ferulago angulata in three developmental stages from two natural habitats of Ilam Province.
Materials and Methods: The extracts were prepared from aerial parts of Ferulago angulate plant. The total phenol and flavonoid contents of extracts were measured spectrophotometrically by using Folin-Ciocalteu reagent and aluminium chloride, respectively. The antioxidant capacity was determined by DPPH method. The results of this method were expressed by calculating the 50% inhibitory concentration (IC50) of the extract in different developmental stages.
Results: The results showed that the highest phenolic and flavonoid content is in the flowering development stage of Kabirkouh habitat. The DPPH scavenging activity results of methanolic extracts showed that the lowest IC50 value (the highest antioxidant activity) and the highest IC50 value (the lowest antioxidant activity) of methanolic extracts were 0.71 and 2.06 mg/ml at developmental flowering and pre-flowering stages, respectively.
Conclusion: All extracts showed antioxidant activity, which is more effective in the developmental flowering stage of Kabirkouh habitat. This plant can be considered an available source of natural antioxidants that can be used in the food and pharmaceutical industries. In the study of two habitats and three developmental stages, Kabirkouh habitat and the flowering developmental stage are suggested as the most suitable habitats and developmental time to collect this medicinal plant.
References
1. Aghajani, A., Mortazavi, S. A., Tabatabai Yazdi, F., Shafafi Zenozian, M., & Saedi Asl, M. R. (2019). Determination of the phenolic content and free radical scavenging activity of extract obtained from chevil (Ferulago angulata) leaves by microwave and maceration. Journal of food science and technology (Iran), 16(86), 119-131 (In Persian).
2. Alirezaie Noghgondar, M., Azizi, M., Neamati, S., Rezvani Moghaddam, P., & Rezazadeh, S. (2016). Variation of some phytochemical compound in shoot and root of Rumex turcomanicus Czerep. at different phenological stages. Journal of Medicinal Plants, 15(58), 25-36 (In Persian).
3. Azizian Shermeh, O., Taherizadeh, M., Valizadeh, M., & Qasemi, A. (2017). Robial and antioxidant activities and determining phenolic and flavonoid contents of the extracts of five species from different families of the medicinal plants grown in Sistan and Baluchestan Province. Journal of Advanced Biomedical Sciences, 7(4), 465-479 (In Persian).
4. Batooli, H., Akbari, M., Yasa, N., Khanavi, M & Tavakoli, S. (2016). Comparison of essential oil composition of Pterocarya fraxinifolia leaves in different phenology stages from Gilan Province. Eco-Phytochemical Journal of Medical Plants, 4, 83-94. [DOI:10.30495/EJMP.2016.694516. (In Persian).]
5. Darderafshi, M., Bahrami, G., Sadeghi, E., Khanahmadi, M., Mohammadi, M., & Mohammadi, R. (2014). The effect of Ferulago angulata essential oil on Staphylococcus aureus during the manufacture and preservation of Iranian white cheese. Iranian Journal of Nutrition Sciences and Food Technology, 8(4), 13-20(In Persian).
6. Esmaeili, S., Hajimehdipoor, H., Ramezani, A., & Mosaddegh, M. (2012). The Cytotoxic Effects of Ferula Persica var. Persica and Ferula hezarlalehzarica against HepG2, A549, HT29, MCF7 and MDBK Cell Lines. Iranian Journal of Pharmaceutical Sciences, 8(2), 115-119 (In Persian).
7. Farjampour, M., Parsai, S., Naghiha, R. & Khajavi, M. (2016). The effect of adding Cavill powder (Ferulago angulata) on performance, blood parameters, intestine microflora and humoral immunity of female broiler chickens. Iranian Veterinary Journal, 12, 90-100 (In Persian).
8. Fazeli-nasab, B., Moshtaghi, N., & Forouzandeh, M. (2019). Effect of solvent extraction on phenol, flavonoids and antioxidant activity of some Iranian native herbs. Journal of Ilam University of Medical Sciences, 27(3), 14-26.
https://doi.org/10.29252/sjimu.27.3.14 [DOI:10.29252/sjimu.27.3.14. (In Persian).]
9. Habibi, H., Mazaheri, D., Hosseini, N. M., Chaeichi, M. R., Tabatabaei, M., & Bigdeli, M. R. (2007). Effect of altitude on essential oil and components in wild Thyme (Thymus kotschyanus Boiss) Taleghan region. Pajouhesh-va-Sazandegi, 19: 2-10 (In Persian).
10. Hosseini, B., Nikkhah Amirabad, H., Fattah, M. & Yort, G. (2017). Effect of altitude and different phonological stages on essential composition and antioxidant activity of Ferula angulata (Schlecht.) Boiss from Dena altitudes. Eco-phytochemical Journal of Medicinal Plants, 5, 16-29 (In Persian).
11. Jafari, M. (2008). The environmental features of the protected area of Manesht and Ghalarang. Department of Environment, Ilam (In Persian).
12. Jafari, N., Naderi, P. & Ebrahimzadeh, M.A. (2015). Evaluation of phenolic content, total flavonoid and survey of antioxidant activity of leaves of Ficus carica and Pterocarya fraxinifolia trees using spectrophotometry and highperformance liquid chromatograph methods. Iranian Journal of Plant Biology, 25, 1-16 (In Persian).
13. Jakovljević, Z. D., Stanković, S.M. & Topuzović, D.M. (2013). Seasonal variability of Chelidonium majus. Secondary metabolites content and antioxidant activity. Experimental and Clinical Sciences Journal, 12, 260-269.
14. Kamali, M., Khosroyar, S., & Jalilvand, M. R. (2014). Evaluation of phenolic, flavonoids, anthocyanin contents and antioxidant capacities of different extracts of aerial parts of Dracocephalum kotschyi. North Khorasan University of Medical Sciences, 6(3), 627-634.
https://doi.org/10.29252/jnkums.6.3.627 [DOI:10.29252/jnkums.6.3.627 (In Persian).]
15. Karimian, V., Vahabi, M.R., Fazilati, M & Soleimani, F. (2013). Chemical composition in two species of Verbascum collected from natural habitats, southern Iran. Journal of Herbal Drugs, 4, 127-132.
16. Kaur, R., Kapoor, K. & Kaur, H. (2011). Plants as a source of anticancer agents. Journal of Natural Product and Plant Resources, 1, 119-124.
17. Mantashlo, J., Deljou, A., & Piri, K. (2017). The study of flavonoids and antioxidant power of etanolic, metanolic, hydroalcholic and etylacetatic extracts of branch and stem bark of Salix alba. Cellular and Molecular Research (Iranian Journal of Biology), 30(3), 295-303 (In Persian).
18. Martz, F., Jaakola, L., Julkunen-Tiitto, R., & Stark, S. (2010). Phenolic composition and antioxidant capacity of bilberry (Vaccinium myrtillus) leaves in Northern Europe following foliar development and along environmental gradients. Journal of chemical ecology, 36, 1017-1028. [DOI:10.1007/s10886-010-9836-9]
19. Mazandarani, M., Momeji, A & Zarghami Moghadam, P. (2012). Evaluation of phytochemical and antioxidant activities from different parts of Nasturtium officinale R. Br. in Mazandaran. Iranian Journal of Plant Physiology, 3, 659-664.
20. Mozaffarian, V. (2008). Flora of Ilam. Contemporary Culture Publications (In Persian).
21. Naghiloo, S., Movafeghi, A., Delazar, A., Nazemiyeh, H., Asnaashari, S. & Dadpour, M.R. (2012). Ontogenetic variation of volatiles and antioxidant activity in leaves of Astragalus compactus lam. (Fabaceae). Experimental and Clinical Sciences Journal, 11, 436-443 (In Persian).
22. Nasibi, F., Kalantari, K. & Rashidi Ravari, A. (2003). Investigation of change in morphological and physiological parameter induced by UV-A, UV-B and UV-C of ultraviolet radiation in colza seedling (Brassica napus). Pajouhesh-va-Sazandegi, 60, 97-103 (In Persian).
23. Pouzesh, H., Tatian, M., Jafarian, Z., Tamartash, R., & Nejad Ebrahimi, S. (2018). Evaluating nutritional indices of Ferulago angulata (Schlecht.) Boiss in different habitats of Kohgiluyeh & Boyer-Ahmad province. Journal of Rangeland, 11(4), 486-498 (In Persian).
24. Rafieirad, M., Eydipour, Z., & Alami Rostami, S. (2018). Anti-depressive effects of hydro-alcoholic extract of Chevilan (Ferulago angulate Boiss.) in adult male wistar rat model of ischemia/ hypoperfusion. Nova Biologica Reperta, 5(2), 137-143.
https://doi.org/10.29252/nbr.5.2.137 [DOI:10.29252/nbr.5.2.137 (In Persian).]
25. Saberi, M., Nik Nahad, H., Heshmati, G., Barani, H. & Shahriari, A. Evaluation of the content and performance of some active ingredients extracts of Citrullus colocynthis organs from two habitats of Sistan and Balochestan Province in different growth stages. Journal of Plant Ecosystem Conservation, 511, 49-63 (In Persian).
26. Sharifi, A., Seifi, T., Mohammadzadeh, A., Hammoun Navard, S. & Pajohi-Alamoti, M.R. (2015). Antibacterial activity of alcoholic extract of Ferulago angolata. Scientific Journal of Ilam University of Medical Sciences, 23, 202-208 (In Persian).
27. Smith, H. (1973). Regulatory mechanisms in the photocontrol of flavonoid biosynthesis. In: Milborrow BV (ed.): Biosynthesis and its control in plants. New York: Academic Press.
28. Stankovic, M. S., Niciforovic, N., Topuzovic, M. & Solujic, S. (2011). Total phenolic content, flavonoid concentrations and antioxidant activity of the whole plant and plant parts extracts from Teucrium montanuml var. montanum, F. supinum (L.) Reichenb. Biotechnology and Biotechnological Equipment, 25, 2222-2227.
https://doi.org/10.5586/asbp.2012.010 [DOI:10.5586/asbp.2012.010.]
29. Toncer, O., Karaman, S. & Diraz, E. (2010). An annual variation in essential oil composition of Origanum syriacum from Southeast Anatolia of Turkey. Journal of Medicinal Plant Research, 11, 1059-1064. [DOI:https://doi.org/10.5897/JMPR09.514.]
30. Yang, F. M., L.F. (2010). Adaptive responses to progressive drought stress in two poplar species originating from different altitudes. Silva Fennica, 44, 23-27.
https://doi.org/10.14214/sf.160 [DOI:https://doi.org/10.14214/sf.160.]
31. Zhu, W., Du, Y., Meng, H., Dong, Y. & Li, L. (2017). A review of traditional pharmacological uses, phytochemistry, and pharmacological activities of Tribulus terrestris. Chemistry Central Journal, 60, 1-16.
https://doi.org/10.1186/s13065-017-0289-x [DOI:https://doi.org/10.1186/s13065-017-0289-x.]
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