دوره 12، شماره 36 - ( زمستان 1399 1399 )
جلد 12 شماره 36 صفحات 89-77 |
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Derakhshan A, Babaeizad V, Panjekeh N, Taheri A. (2020). Study of Biochemical and Molecular Changes of Iranian Rice Cultivars in Interaction with Bacterial Pathogen Xanthomonas oryzae pv. oryzae Causes Leaf Blight Disease. J Crop Breed. 12(36), 77-89. doi:10.52547/jcb.12.36.77
URL: http://jcb.sanru.ac.ir/article-1-1142-fa.html
URL: http://jcb.sanru.ac.ir/article-1-1142-fa.html
درخشان احمد، سالاری محمد، بابایی زاد ولی اله، پنجه که ناصر، طاهری عبدالحسین. ارزیابی و مطالعه تغییرات بیوشیمیایی و مولکولی چند رقم برنج ایرانی در تعامل با بیمارگر باکتریایی Xanthomonas oryzae pv. oryzae عامل بیماری سوختگی برگ پژوهشنامه اصلاح گیاهان زراعی 1399; 12 (36) :89-77 10.52547/jcb.12.36.77
1- دانشگاه زابل
2- دانشگاه علوم کشاورزی و منابع طبیعی ساری
3- داشنگاه علوم کشاورزی و منابع طبیعی گرگان
2- دانشگاه علوم کشاورزی و منابع طبیعی ساری
3- داشنگاه علوم کشاورزی و منابع طبیعی گرگان
چکیده: (2886 مشاهده)
سوختگی باکتریایی برنج (Xoo) Xanthamonos oryzae pv. oryzae یکی از مخربترین بیماریهای باکتریایی برنج در برخی از مناطق کشت برنج در دنیا بویژه مناطق گرمسیری آسیایی میباشد. کارایی پایین روش هایی مدیریت بیماری بخصوص روش شیمیایی سبب شده تا بیشتر تحقیقات روی ارقام مقاوم و درک مکانیسم های مقاومت از طریق مطالعه تعاملات بیوشیمیایی و شناخت ژنهای مقاوم در مدیریت برنامه کاربردی قرار گیرد. این پژوهش با هدف ارزیابی مقاومت 24 رقم تجاری برنج ایرانی در مقابل باکتری عامل سوختگی و مطالعه برخی مکانسیم مقاومت در سطوح بیوشیمیایی و الگوی تظاهر ژن PAL به روش QRT-PCR انجام گرفت. نتایج ارزیابی گلخانهای نشان داد ارقام مختلف در توسعه طول لکه در برگ به عنوان مهمترین صفت ارزیابی بیماری اختلاف دارند. رقم تجاری خزر و نعمت در بالاترین سطح مقاومت و رقم طارم محلی و دیلمانی بیشترین حساسیت را بر اساس شاخص ارزیابی (مقاومت کمی) نسبت به این بیماری نشان دادند. ارزیابی بیوشیمیایی آنزیم های کاتالاز، گایکول پراکسیداز، سوپراکسید دیسموتاز و فنل کل در رقم مقاوم خزر و حساس طارم محلی نشان از روند فعالیت بالای این آنزیمها در رقم مقاوم در مقایسه با رقم حساس در ساعات اولیه پس از مایه زنی داشته که اختلاف بین رقم مقاوم و حساس در سطح یک درصد معنی دار می باشد. نرخ بیان ژن PAL نیز در رقم مقاوم خزر در ساعات اولیه پس از مایه زنی نسبت به رقم حساس بطور قابل ملاحظهای بالاتر بوده است. در مجموع به نظر می رسد القای ژن PAL و بدنبال آن افزایش تجمع آنزیمهای اکسیدانی و آنتی اکسیدانی در بروز مقاومت رقم خزر نسبت به رقم حساس طارم محلی بخشی از ساز وکار دفاعی برنج در مقابل باکتری Xoo می باشد.
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