Volume 11, Issue 31 (12-2019)
J Crop Breed 2019, 11(31): 124-133 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mohebodini M, Mirzadeh Qassabeh R, behnamian M, Chamani E, Fathi R. (2019). Investigation of the Effect of Plant Growth Regulators on Micropropagation and Regeneration of Thymus Daenensis Celak. J Crop Breed. 11(31), 124-133. doi:10.29252/jcb.11.31.124
URL: http://jcb.sanru.ac.ir/article-1-1031-en.html
URL: http://jcb.sanru.ac.ir/article-1-1031-en.html
University of Mohaghegh Ardabili
Abstract: (2984 Views)
Daenensis thyme (Thymus daenensis Celak) is one of the most important medicinal plants that belongs to the family of Labiatae. The leaves and flowering branches of this plant have a number of important compounds such as Thymol, Carvacrol, Btaptyn and Parasymyn. The present study was performed in order to determine the optimum of plant growth regulators (auxin and cytokinin) for production of in vitro plantlet using apical bud and hypocotyl explants. The seeds after sterilization were germinated on MS medium. The explants were cultured on the medium containing various concentrations of BAP and Kin for study of in vitro micropropagation. Hypocotyl explants were cultured on the medium containing various combinations of BAP and NAA for callus induction and shoot regeneration. In study of micropropagation, the mean comparison showed the highest number of shoot, leaf and bud obtained in MS medium containing 2.5 mg l-1 Kin in the apical explants. In study of regeneration, the mean comparison showed the highest percentage of shoot regeneration and mean of shoot number were obtained on MS medium supplemented with 1.5 mg l-1 BAP + 0.6 mg l-1 NAA in hypocotyl explants. Overall, Kin was most effective plant growth regulator on micropropagation and comination of BAP and NAA had most effect on regeneration of Daenensis thyme.
Type of Study: Research |
Subject:
بيوتكنولوژي گياهي
Received: 2019/02/12 | Revised: 2020/09/16 | Accepted: 2019/05/13 | Published: 2019/12/4
Received: 2019/02/12 | Revised: 2020/09/16 | Accepted: 2019/05/13 | Published: 2019/12/4
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
