Journal of Crop Breeding
پژوهشنامه اصلاح گیاهان زراعی
jcb
Agriculture
http://jcb.sanru.ac.ir
1
admin
2228-6128
2676-4628
10.61186/jcb
fa
jalali
1395
2
1
gregorian
2016
5
1
8
18
online
1
fulltext
fa
تجزیه همبستگی برخی صفات زراعی با نشان گرهای ریزماهواره برای انتخاب کلونهای پرقند نیشکر در استان خوزستان
Correlation Analysis of Some Agronomic Traits by using Microsatellite Markers for Select High Sugar Sugarcane Clones in Khuzestan Province
اصلاح نباتات
General
پژوهشي
Research
<p class="Style1" dir="RTL" style="margin: 0cm 0cm 0pt;"><strong><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">روش تجزیه هم‏بستگی، امکان شناسایی اولیه و سریع ژنهای کنترلکننده صفات کمی را ممکن میسازد. در این تحقیق ارتباط بین 14 صفت زراعی و 96 باند حاصل از 20 جفت آغازگر ریزماهواره روی 26 کلون و 4 رقم تجاری از راه هم‏بستگی کانونیکال مطالعه شد. میانگین تعداد آلل­ها 15/5 آلل برای هر مکان ریزماهواره بود. میزان محتوای اطلاعات چندشکلی (</span><font size="2"><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">PIC</span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">)</span><span dir="LTR" lang="AR-SA" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra"; mso-ansi-language: EN-US;"> </span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">آغازگرها بین 19/0 تا 71/0 متغیر بود. نشان گرهای 62734</span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">AFO</span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">، 688</span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">UGSM</span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;"> و 2</span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">UGSM</span><span lang="X-NONE" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;"> </span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">با بیشترین محتوای اطلاعات چندشکلی آغازگرها، بیشترین شاخص چندشکلی را نشان دادند و توانستند بهتر از بقیه نشانگرها فاصله ژنتیکی ارقام را مشخص کنند، در حالیکه نشانگرهای 31</span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">UGSM</span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;"> و 6</span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">SGM</span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;"> با کمترین مقدار شاخص چندشکلی، توانایی جداسازی ژنوتیپها را نداشتند. نتایج تجزیه هم‏بستگی کانونیکال نشان داد که بین تعداد زیادی از مکانهای ژنی و حداقل یکی از صفات زراعی به جز درصد شربت و درصد خلوص شربت ارتباط معنیداری وجود دارد. بنابراین احتمالا میتوان از این مکانهای ژنی برای انتخاب بهینه صفات مورفولوژیک در نیشکر</span><span dir="LTR" lang="AR-SA" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";"> </span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">استفاده کرد. بیشترین هم‏بستگی مربوط به صفت عملکرد شکر و عملکرد نی توسط نشانگر </span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">CG</span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">226</span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">SMC</span><span lang="X-NONE" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;"> </span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">به</span><span dir="LTR" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra"; mso-ansi-language: EN-US;">­</span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">ترتیب 25 و 26 درصد و برای صفت درصد قند قابل استحصال توسط نشانگر 62734</span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">AFO</span><span lang="X-NONE" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;"> </span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">(12 درصد) به دست آمد. همچنین برخی از نشانگرها با بیش از یک صفت ارتباط نشان میدهند که بیان گر این است که این صفات پیوستگی بسیار نزدیکی با همدیگر داشته و یا احتمالا تحت تاثیر ژنهای چنداثره قرار دارند. در مطالعات آینده، از باند نشانگرهای شناسایی شده که هم‏بستگی (</span><span dir="LTR" lang="X-NONE" style="color: black; font-family: "Times New Roman","serif"; mso-bidi-font-family: "2 Mitra";">r2</span><span lang="AR-SA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-ansi-font-size: 8.0pt;">) بالایی با صفات زراعی دارند، میتوان برای انتخاب والدین تلاقیها و اصلاح ارقام پرقند استفاده کرد.</span></font><span lang="FA" style="color: black; line-height: 80%; font-family: "2 Mitra"; font-size: 10pt; mso-ascii-font-family: "Times New Roman"; mso-hansi-font-family: "Times New Roman"; mso-bidi-language: FA; mso-ansi-font-size: 8.0pt;"><!--stripped--><!--stripped--></span></strong></p>
<p>Correlation analysis method makes possible early and rapid identification of genes controlling quantitative traits. In this research, the relationship between 14 agronomic traits and 96 bonds obtained from 20 pairs of microsatellite primers on 26 clones and four commercial cultivars has been studied by Canonical correlation. The average number of alleles was 5.15 for each microsatellite location. The polymorphic information content (PIC) of primers was variable between 0.19 and 0.71. Markers of AFO62734, UGSM688 and UGSM2 with the most polymorphic information content of the primers showed the most polymorphic index and could identify the genetic distance of numbers better than the rest of the markers. While markers of UGSM31and SGM6 with lowest amount of Polymorphic index were not able to isolate genotypes. The results of canonical correlation analysis indicated that there is significant relationship between large number of loci and at least one of the agronomic traits except the percentage of juice and the percentage of juice purity. Therefore, there is probability of using these loci to select the optimal of morphological traits in sugarcane. The most correlation related to sugar yield trait and straw yield were obtained by marker of SMC226CG respectively 25 and 26 percent and for percentage trait of white sugar by marker of AFO62734 (%12). Also some markers showed more than one association trait which indicates that these traits have close conjunction with each other or probably are under the influence of multi-effect genes. In future studies, the band of identified markers which have high correlation (r2) with agronomic traits can be used to select the parent crosses and improve high sugar varieties.</p>
نیشکر, قند, ریزماهواره, صفات زراعی, همبستگی کانونیکال
traits, Canonical Correlation, Microsatellite, Sugar, Sugarcane
104
111
http://jcb.sanru.ac.ir/browse.php?a_code=A-10-1-187&slc_lang=fa&sid=1
سارا
عصاره زادگان دزفولی
10031947532846004280
10031947532846004280
Yes
خلیل
عالمی سعید
10031947532846004281
10031947532846004281
No
رضا
مامقانی
10031947532846004282
10031947532846004282
No
زهرا
خدارحمپور
10031947532846004283
10031947532846004283
No