Volume 10, Issue 26 (9-2018)                   jcb 2018, 10(26): 22-31 | Back to browse issues page


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Naeemi T, Fahmideh L, Fakheri B A. (2018). The Impact of Drought Stress on Antioxidant Enzymes Activities, Containing of Proline and Carbohydrate in Some Genotypes of Durum Wheat (Triticum turgidu L.) at Seedling Stage . jcb. 10(26), 22-31. doi:10.29252/jcb.10.26.22
URL: http://jcb.sanru.ac.ir/article-1-661-en.html
Abstract:   (3778 Views)

Drought and changes in weather conditions, cause damage and reduce their agricultural products. The fact that durum wheat, after common wheat or Simultaneous with that, is one of the major sources of food supplies, makes it a necessity to identify its drought tolerant species.This article reports an experiment on the impact of different levels of drought ( 5,10,20, 25 percent of field capacity) on activity of antioxidant enzymes, including Catalase (CAT), Poly phenol oxidase (PPO), Ascorbate peroxidase (APX), Guaiacol peroxidase (GPX), and also some photosynthetic pigments ( Chlorophylls a, b, total Chlorophyll and Carotenoid ), as well as some osmosic regulators include Carbohydrates and Proline in durum wheat genotypes (Shabrang, Behrang, Karkhe, Aria, Dena ). This experiment was designed as a factorial randomized blocks with three repeats, conducted at the Institute for Biotechnological Research in the University of Zabol. Following planting the seeds in pots, the drought stress was introduced at the tillering stage, after which the attributes of the plants were measured. The results of analysis of variance demonstrated that the impact of genotype, drought stress, and the interaction of stress and drought were statistically significant at 1 percent and 5 percent in all the attributes under investigation. The impact of genotype and drought stress on polyphenol oxidase trait was not significant. The most significant effects observed were those of 5 percent watering level of the capacity on antioxidant activity and osmos regulator. The results of this study indicated that in the conditions of this experiment, Behrang and Karkheh genotypes determined the highest level of photosentises pigments and Shabrang genotype have most level of antioxidant activity and Karkheh genotype have highest level of proline and carbohydrate contain, identifying it as a drought tolerant species.
 

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Type of Study: Research | Subject: Special
Received: 2016/11/14 | Revised: 2018/09/26 | Accepted: 2017/09/10 | Published: 2018/09/26

References
1. Agarwal, S. and V. Pandey. 2004: Antioxidant enzyme responses to Nacl stress in Cassia angustifolia. Journal of Biology Plant, 48: 555-560. [DOI:10.1023/B:BIOP.0000047152.07878.e7]
2. Ahmadi, A. and A. Ceiocemardeh. 2004. Effect of drought stress on soluble carbohydrate, chlorophyll and Proline in four adopted wheat cultivars with various climate of Iran. Journal of Agricultur Science, 35: 753-763 )In Persian(.
3. Alvesda Costa, P.H., A.D. Azevedo Neto, M. Alves Bezerra, J. Tarquinio paisco and E. Gomes- Filho. 2005. Antoxidative-enzymatic system of two sorghum genotypes differing in salt tolerance. Plant Physiology, 17: 353-361. [DOI:10.1590/S1677-04202005000400003]
4. Amiri Deh Ahmadi, S.R. and E. MohamadiGanjeali. 2010. Effects of drought stress on morphological characteristics and yield components in different phenological stages of chickpea (Cicer arietinum L.) greenhouse conditions. Journal of Agricultural Research, 8: 166-157 )In Persian(.
5. Arnon, A.N. 1967. Method of extraction of chlorophyll in the plants. Agronomy Journal, 23: 112-121.
6. Ashraf, M.Y., A.R. Azmi, A.H. Khan and S.A. Ala. 1994. Effect of water on total phenols, peroxidase activity and chlorophyll content in wheat. Acta Phsiologiae Plantarum, 16: 185-191.
7. Bandyopadhyay, P., X. Linehan-Stieers, C.B.T. Kren and C.J. Steer. 1999. Nucleotide exchange in genomic DNA of rat hepatocytes using RNA/DNA oligonucleotides. Targeted delivery of liposomes and polyethylene mine to the asialoglyco protein receptor. Journal of Biological Chemistry, 15: 10163-10172. [DOI:10.1074/jbc.274.15.10163]
8. Bates, S., R.P. Waldern and E.D. Teare. 1973. Rapide determination of free proline for water stress studies, Plant and Soli, 39: 205-207. [DOI:10.1007/BF00018060]
9. Beers, G.R. and I.W. Sizer. 1952. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Biology Chemical, 195: 133-140.
10. Blum, A., G. Gozlan and J. Mayer. 1981. The Manifestation of Dehydration Avoidance in Wheat Breeding Germplasm. Crop Science, 21: 495-499. [DOI:10.2135/cropsci1981.0011183X002100040004x]
11. Bonwell, E.S., T.L. Fisher, A.K. Fritz and D.L. Wetzel. 2008. Determination of endosperm secondary structure in hard wheat breeding lines using synchrotron infrared crospectroscopy protein. Journal of Vibrational Spectroscopy, 48: 76-81. [DOI:10.1016/j.vibspec.2008.04.002]
12. Delauney, A.J. and D.P.S. Verma. 1993. Proline biosynthesis and osmoregulation in plants. Plant Journal, 4: 215-223. [DOI:10.1046/j.1365-313X.1993.04020215.x]
13. Esfandiari, E., A. Javadi, M. Shokrpour and F. Shekari. 2011. The effect of salt stress on theantioxidant defense mechanisms on wheat seedling. Fresenius Environmental Bulletin, 20: 2021-2036 (In Persian).
14. Esfandiari, E.A., M.R. Shakiba, S.A. Mahboob, H. Alyari and S. Shahabivand. 2009. The effect of water stress on the antioxidant content, protective enzyme activities, proline content and lipid peroxidation in wheat seedling. Pakistan Journal of Biological Sciences, 11: 1916-1922 (In Persian). [DOI:10.3923/pjbs.2008.1916.1922]
15. Fedine, L.S. and A.V. Popova. 1996. Photosynthesis, photorespiration and proline accumulation in water-stressed pea leaves. Crop Science, 32: 213-220.
16. Fielding, J.L. and J. Hall. 1978. A biochemical and cytochemical Study of peroxidase a ctivity in root pea. Journal of Experimental Botany, 29: 98-989. [DOI:10.1093/jxb/29.4.983]
17. Gharbi, A., A. Rashidin, S. Tarynzhad and Q. Chlbyyany. 2013. Salinity and drought tolerance of durum wheat lines under greenhouse conditions. Journal of Crop Eco Physiology, 4: 393-410 (In Persian(.
18. Gosset, D.R., E.P. Millhollon and M.C. Lucas. 1994. Antioxidant response to NaCl stress in salt-tolerant and salt sensitive cultivars of cotton. Crop Science, 34: 106-714. [DOI:10.2135/cropsci1994.0011183X003400030020x]
19. Gregersen, P.L. and P.B. Holm. 2007. Transcriptome analysis of senescence in the flag leaf of wheat (Triticum aestivum L.). Journal of Plant Biotechnology, 5: 192-206. [DOI:10.1111/j.1467-7652.2006.00232.x]
20. Gressel, J., E. Galun, C. H.Foyer and P. M.Mullineaux. 1994. Genetic controls of photo oxidant tolerance. Causes of photooxidative stress and amelioration of defense systems in plants, 237-273
21. Hassanpour Lesko Kalaye, K., J. Ahmadi, J. Daneshian and P. Hatem. 2015. Determine the changes in chlorophyll, protein and antioxidant enzymes in durum wheat under drought stress. Journal of modified crop plants, 7: 76-87 )In persian(.
22. Heidari, M. and F. Tarahomi. 2010. Effect of different levels of salinity on physiological reactions and Zn uptake of sodium and potassium. Journal of environmental stress on Crop Science, 3: 83-93 )In persian(.
23. Hendry, G. 1993. Evolutionary origins and natural functions of fructanc. New Phytologist, 123: 3-14. [DOI:10.1111/j.1469-8137.1993.tb04525.x]
24. Hernandez, J.A., M. Angeles Ferrer, A. Jimenez, A. Ros Barcelo and F. Sevilla. 2001. Antioxidant Systems and O2.−/H2O2 Production in the Apoplast of Pea Leaves. It's Relation with Salt-Induced Necrotic Lesions in Minor Veins. Plant Physiol. 2001 Nov, 127: 817-831. [DOI:10.1104/pp.010188]
25. Hissao, T. 1973. Plant responses to water stress. Ann. Rev. Plant Physiol, 24: 519-570. [DOI:10.1146/annurev.pp.24.060173.002511]
26. Irrigoyen, J.H., D.W. Emerich and M. Sanchez Diaz. 1992. Water stress induced changes in concentration of proline and total soluble sugars in nodulated alfalfa plant. Physiologic Pantarum, 84: 55-66. [DOI:10.1034/j.1399-3054.1992.840109.x]
27. Jagtap, V., S. Bhargava, P. Stredo and J. Feirabend. 1998. Comparative effect of water, salt and light stresses on photosynthetic reactions in (Sorghurm biocolor L. Moench). Journal of Experimental Botany, 49: 1715-1721. [DOI:10.1093/jexbot/49.327.1715]
28. Janovitz-Klapp, A.H., F.C. Richard. P.M.J.Goupyand and J. Nicolas. 1990. Inhibition studies on apple polyphenol oxadase. Journal of Agricultural Food Chemistry, 38: 926-931. [DOI:10.1021/jf00094a002]
29. Kamrava, S., N. Babaeian Jolodar and N. Bagheri. 2017. Evaluation of Drought Stress on Chlorophyll and Proline Traits in Soybean Genotypes. Journal of Crop Breeding, 9(23): 95-104 )In Persian(.
30. Kriedmann, P.E. 1980. Stomatal and photosynthetic limitations to leaf growth. Plant physiology, 13: 145-31.
31. Kuznestov, VI.V. and N.I. Shevyakova. 1999. Prolin under stress: metabolism and regulation. Russian jornal of Plant Physiology, 46: 274-286.
32. Lichtenthaler, H. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes Methods in Enzymology, 148: 350-382. [DOI:10.1016/0076-6879(87)48036-1]
33. Loggini, B., A. Scartazza, E. Brugnoli and F. NavariIzzo. 1999. Antioxidative Defense System, Pigment Composition, and Photosynthetic Efficiency in Two Wheat Cultivars Subjected to Drought. Plant Physiology, 119: 1091-1099. [DOI:10.1104/pp.119.3.1091]
34. Maali-Amiri, R., I.V. Goldenkova-Pavlova, V. Pchelkin, V.D. Tsydendambaev, A.G. Vereshchagin, A.N. Deryabin, T.I. Trunova, D.A. Los and A.M. Nosov. 2007. Lipid fatty acid composition of potato plants transformed with the Δ12-desaturase gene from cyanobacterium. Russian Journal of Plant Physiology, 54: 678-685. [DOI:10.1134/S1021443707050056]
35. Malek Ahmadi, F., K.H.M. Station and A. Taher Race. 2005. Ghrqa no stress on the accumulation of some minerals and induce oxidative stress in plants pepper (capsicum annum L.), Fourth National Conference on Biotechnology Iran, Kerman, International Center for Advanced Science and Technology and Environmental Sciences, 104-214 )In Persian(.
36. Masinovsky, Z., G.I. Lozovaya and A.A. Sivash. 1992. Some aspects of the early evolution of photosynthesis. Advances in Space Research, 12: 199-205. [DOI:10.1016/0273-1177(92)90173-U]
37. Michalak, P. 2006. RNA world-the dark matter of evolutionary genomics. Journal compilation European society for Evolutionary Biology, 19: 1768-1774. [DOI:10.1111/j.1420-9101.2006.01141.x]
38. Mihailović, N., M. Lazarević, Z. Dželetović, M. Vučković and M. Đurđević. 1997. Chlorophyllase activity in wheat, (Triticum aestivum L.). Leaves during drought and its dependence on the nitrogen ion form applied. Plant Science, 129: 141-146. [DOI:10.1016/S0168-9452(97)00189-1]
39. Mittal, S., N. Kumari and V. Sharma. 2012. Differential response of salt stress on Brassica juncea: Photosyntheticperformance, pigment, proline, D1 andantioxidant enzymes. Plant Physiolgy Biochemical, 54: 17-26. [DOI:10.1016/j.plaphy.2012.02.003]
40. Moradi, A., A. Ahmadi and M. Judy. 2005. Response of photosynthesis and stomatal conductance 0. gram Severe and Moderate Drought stress at different growth stages. First Conference on bean National Institute of Plant Sciences Plant Sciences University of Mashhad, 272-268 )In Persian(.
41. Ranney, T.G., N.L. Bassuk and T.H. whitlow. 1991. Osmotic adjustment and solute contributes in leaves and roots of water-stressed cherry (prunus) trees. Journal of the American society for Horticultural Science, 116: 684-688. [DOI:10.21273/JASHS.116.4.684]
42. Shahriari, P., A. Mirshams Kakhaki and M. Amini. 2012. Molecular detection of enzyme polyphenol oxidase in a number of common wheat varieties in Iran marker by STS. Iran's third national conference on agricultural biotechnology of plant, animal and industrial, Mashhad, Ferdowsi University of Mashhad, 45 pp (In Persian(.
43. Shokrpur, M. and AS. Esfandiari. 2014. Grvhbndy different varieties tolerant to salinity based on biochemical and physiological indices. Journal of Crop Breeding, 14: 54-65 )In Persian(.
44. Syosamardha, A.A., K. Ahmadi, V.H. Postini and H. Ebrahimzadeh. 2004. Factor controlling stomatalaperture and photosynthesis and its relationship with drought resistance in wheat cultivars. Journal of Agricultural Science, 35: 93-106 (In Persian).
45. Tarahomi, P., D. Lahooti and P. Abbasi. 2010. Effects of drought stress on soluble sugars, chlorophyll and potassium S. leriifolia (Salvia leriifolia Benth). Journal of Biological Sciences, 3: 1-7 )In Persian(.
46. Verma, S. and R.S. Dubey. 2003. Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Science, 164: 645-655 [DOI:10.1016/S0168-9452(03)00022-0]
47. Xue, T., X. Li, W. Zhu, C.H. Wu, G. Yang and C.H. Zheng. 2008. Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast. Journal of Experimental Botany, 60: 339-349. [DOI:10.1093/jxb/ern291]
48. Yan, B., Q. Dai, X. Liu, S.H. Huang and Z. Wang. 1996. Plant and Soil Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves. Springer, 179: 261-268. [DOI:10.1007/BF00009336]
49. Yoshimura, K., Y. Yabute. T. Ishikawa and S. Shigeoka. 2000. Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. Plant Physiology, 123: 223-233. [DOI:10.1104/pp.123.1.223]

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