Volume 8, Issue 18 (5-2016)                   jcb 2016, 8(18): 204-211 | Back to browse issues page


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(2016). Evaluation of Phylogenetic Relationships for Waxy Genes in Wild and Cultivated Wheat using Multiplex-PCR. jcb. 8(18), 204-211. doi:10.29252/jcb.8.18.204
URL: http://jcb.sanru.ac.ir/article-1-657-en.html
Abstract:   (3249 Views)

Waxy proteins are responsible for amylase synthesis in wheat seeds, being encoded by three waxy genes (Wx-A1, Wx-B1 and Wx-D1) in hexaploid wheat which have an important role in starch quality. The purpose of this study was to investigate phylogenetic relationship between waxy genes in wild and cultivated wheat using Multiplex-PCR. To this end, 71 populations from 8 Aegilops and 4 wheat wild spices ‘were studied. Clustering results showed that the samples were divided in two main groups. Diploid samples with A, B and D genomes located in the same group but tetra and hexaploid wheat located in the other group. Also, Biplot results showed that Aegilops with A and D genomes had the greatest genetic affinity for waxy genes. Multiplex-PCR technique is able to identify three waxy genes simultaneously in different populations of wheat which contributed substantially to breeder in cost and time.

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Type of Study: Research | Subject: General
Received: 2016/11/8 | Accepted: 2016/11/8 | Published: 2016/11/8

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