Volume 10, Issue 25 (6-2018)                   jcb 2018, 10(25): 152-158 | Back to browse issues page

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Bagherikia S, Pahlevani M, Bagherikia S, Zenalinezhad K. (2018). Probit Analysis to Determine of the Most Appropriate Dose of Gamma Irradiation in Wheat Mutation Breeding. jcb. 10(25), 152-158. doi:10.29252/jcb.10.25.152
URL: http://jcb.sanru.ac.ir/article-1-632-en.html
Probit Analysis to Determine of the Most Appropriate Dose of Gamma Irradiation in Wheat Mutation Breeding
Saeed Bagherikia , Mohammadhadi Pahlevani , Saeed Bagherikia , Khalil Zenalinezhad
Plaant Breeding & Biotechnology Department, Gorgon University of Agricultural Sciences & Natural Resources
Abstract:   (3698 Views)

Identification of the appropriate dose for mutagen material is the most important step in setting experiment and creating mutant genetic material. According to a definition the most appropriate dose of mutagen is a dose that causing a reduction of 50% survival or 30% growth, compared to control. The aim of this study was to evaluate the effect of different doses of gamma radiation (0 as a control, 100, 200, 300 and 400 Gary) on the early growth characteristics for determining the appropriate dose of gamma irradiation in wheat cultivar Sardari. Therefore, an experiment was conducted based on completely randomized design with three replications at the laboratory and greenhouse. Data analysis of seed germination experiment showed significant difference in all traits including rootlet length, stemlet length, fresh weight of rootlet, fresh weight of stemlet, dry weight of rootlet and dry weight of stemlet, the exception of germination rate. Analysis of data obtained from a greenhouse, also showed significant difference in plant height and survival percentage. Based on the best fitted regression model and probit analysis determined a dose causing reduction of 50% survival or 30% growth, compared to control. It was in the range between 200 to 300 Gary, for Sardari cultivar. It could help to create adequate genetic diversity by applying this range of gamma radiation, for using in the wheat mutation breeding programs.
 
 

Keywords: Cobalt 60, Gamma Irradiation, Genetic Diversity, Mutation Breeding, Regression Model, Wheat
Full-Text [PDF 605 kb]   (1760 Downloads)    
Type of Study: Research | Subject: اصلاح موتاسيوني
Received: 2016/11/6 | Revised: 2018/07/21 | Accepted: 2016/12/24 | Published: 2018/07/8
References
1. Borzouei, M., H. Kafi, B. Khazaei, M. Naseriyan and A. Majdabadi. 2010. Effects of gamma radiation on germination and physiological aspects of wheat (Triticum aestivum L.) Seedlings. Pakestan Journal Botany, 42(4): 2281-2290.
2. Chaudhuri, K.S. 2002. A simple and reliable method to detect gamma irradiated lentil (Lens culinaris Medik.) seeds by germination efficiency and seedling growth test. Radiation Physics and Chemistry, 64(2): 131-136. [DOI:10.1016/S0969-806X(01)00467-4]
3. FAO/IAEA Database of Mutant Variety and Genetic Stock: http://mvgs.iaea.org.
4. FAO; Food and Agriculture Organization, Available http://faostat.fao.org. Last accessed 1 October 2016.
5. Hall, E.J., R. Oliver, B.J. Shepstone and J.S. Bedford. 2003. On the population kinetics of the root meristem of Vicia faba exposed to continuous irradiation. Radiation Research, 27(4): 597-603. [DOI:10.2307/3571843]
6. Kia, M., N.A. Babaeian Jelodar and N.A. Bagheri. 2010. Study on Salt Tolerance of Gamma Ray Induced Mutants in 032 Soybean Cultivar in Greenhouse Condition. Journal of Crop Breeding, 2(5): 47-56 (In Persian).
7. Kiong, A.L.P., A.G. Lai, S, Hussein and A.R. Harun. 2008. Physiological responses of Orthosiphon stamineus plantlets to gamma irradiation. American-Eurasian Journal of Sustainable Agriculture, 2(2): 135-149.
8. Kodym, A., R. Afza, B.P. Forster, Y. Ukai, H. Nakagawa and C. Mba. 2012. Methodology for physical and chemical mutagenic treatments. In: Shu, Q.Y., B.P. Forster and H. Nakagawa (eds.) Plant mutation breeding and biotechnology. 169-180 pp., CABI, Oxford, UK. [DOI:10.1079/9781780640853.0169]
9. Majd, F. and M.R. Ardakani. 2010. Nuclear techniques in agriculture sciences, 2nd edn. Univerity of Tehran Press, Tehran, Iran, 381 pp (In Persian).
10. Mba, C. and Q.Y. Shu. 2012. Gamma irradiation. In: Shu, Q.Y., B.P. Forster and H. Nakagawa (eds.) Plant mutation breeding and biotechnology. 91-98 pp., CABI, Oxford, UK. [DOI:10.1079/9781780640853.0091]
11. Mousavi Shalmani, A.M., B. Naserian Khiabani, H. Ahari Mostafavi. M. Heidarzadeh. and A. Majdabadi. 2009. Nuclear agriculture (from science to practical aspect). 1st edn. Nuclear Science and Technology Institute, Karj, Iran, 518 pp (In Persian).
12. Nakagawa, H. 2009. Induced mutations in plant breeding and biological researches in Japan. Crops, 242(188): 48-54.
13. Preussa, S.B. and A.B. Britta. 2003. A DNA-damage-induced cell cycle checkpoint in Arabidopsis. Genetics, 164(1): 323-34.
14. Samadi Gorji, M., A. Zaman Mirabadi, V. Rammeah, aliollah, M. Hasanpour and A. Esmailifar. 2015. Evaluation of Agronomic Traits of Mutants Induced by Gamma Irradiation in PF and RGS003 Varieties of Rapeseed (Brassica napus L.). Journal of Crop Breeding, 7(15): 135-144 (In Persian).
15. Sikder, S., P. Biswas, P. Hazra, S. Akhtar, A. Chattopadhyay, A.M. Badigannavar and S.F. D'Souza. 2013. Induction of mutation in tomato (Solanum lycopersicum L.) by gamma irradiation and EMS. Indian Journal of Genetics and Plant Breeding, 73(4): 392-399. [DOI:10.5958/j.0975-6906.73.4.059]
16. Singh, N. and H. Balyan. 2009. Induced mutations in bread wheat (Triticum aestivum L.) CV." Kharchia 65" for reduced plant height and improve grain quality traits. Advances in Biological Research, 3(5-6): 215-221.
17. van Harten, A.M. 1998. Mutation breeding: theory and practical applications. 1st edn. Cambridge University Press, Uk, 338 pp.
18. Wi, S.G., B.Y. Chung, J.S. Kim, J.H. Kim, M.H. Baek, J.W. Lee and Y.S. Kim. 2007. Effects of gamma irradiation on morphological changes and biological responses in plants. Micron, 38(6): 553-564. [DOI:10.1016/j.micron.2006.11.002]
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