Genetic diversity of 45 wheat varietions were studied using AFLP markers. From 11 primer combinations of PstI and Mse1for obtaining AFLP polymorphism, six primer combinations (P-CGA/M-CTA, P-ATG/M-CTG, P-ATG/M-CAT, P-AGT/M-CTA, P-AGT/M-CAG and P-ATG/M-CAG) produced polymorphic bands. Totally, 328 bands be produced that 207 bands (63.10%) were polymorphic, which showing high polymorphism among genotypes. PIC was variable between 0.39 in P-AGT/M-CAG and 0.44 in P-ATG/M-CT. According to Nei’s gene index and Shanon’s information index, the highest and the lowest genetic diversity within genotypes on the basis primer combinations were obtained in P-ATG/MM-CAT and P-CGA/M-CTA primer combinations. Cluster analysis based on furthest neighbors linkage and Nei's genetic distance categorized the genotypes into six clusters. The highest and lowest number of genotypes situated in sixth (41.86%) and fourth (4.65%) clusters, respectively. The similarity's range based on Dice similarity coefficient changed between 0.57-0.99. Arta and Ghods had the highest genetic similarity (0.99) and Azadi and Koohdasht owned the lowest genetic similarity.