Volume 8, Issue 20 (3-2017)                   jcb 2017, 8(20): 132-124 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Elyasi L, Mehrabi A, Seyedi M, Safari Z. (2017). Optimization of Callus Culture of Satureja Bachtiarica L. using Different Explants and Concentrations of Growth Regulators. jcb. 8(20), 132-124.
URL: http://jcb.sanru.ac.ir/article-1-363-en.html
Ilam University
Abstract:   (3901 Views)

Satureja bachtiarica L, an important medicinal plant from Lamiaceae family is valuable because of its medical compounds. Optimization of in vitro culture through callogenesis is proposed to be an alternative for vegetative propagation. In present study, an efficient protocol has been developed for callus induction of S. bachtiarica using different explants and concentrations of plant growth regulators on MS basal medium. Results indicated that the highest rate of callogenesis (16.82±0.94 days after culture) and callus volume (14.17±0.47) were achieved from internodes explants. On the other hand, for percentage of callus induction, shoot tips, internodes and nodal segments explants were more efficient than that of leaves explants. According to the results, the nodal segments cultured on medium supplemented with 1 mgl-1 BAP plus 1 mgl-1 NAA led to a remarkable decrease in induction rate of calli. Maximum callus volume was obtained from internodes explants cultured on medium supplemented with 0.5 mgl-1 BAP plus 0.5 mgl-1 NAA.

Full-Text [PDF 635 kb]   (2078 Downloads)    
Type of Study: Research | Subject: بيوتكنولوژي گياهي
Received: 2015/02/17 | Revised: 2017/02/28 | Accepted: 2015/09/20 | Published: 2017/02/28

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Crop Breeding

Designed & Developed by : Yektaweb