دوره 9، شماره 22 - ( تابستان 1396 )
جلد 9 شماره 22 صفحات 124-117 |
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Mehrabioun Mohammadi M, Babaeizad V, Rahimian H, Ebrahim Nejad S. (2017). Screening of some Barley Lines Against Powdery Mildew Agent and Considering of NH1 and Several Pathogenesis Related Genes in Disease Resistance. J Crop Breed. 9(22), 117-124. doi:10.29252/jcb.9.22.117
URL: http://jcb.sanru.ac.ir/article-1-837-fa.html
URL: http://jcb.sanru.ac.ir/article-1-837-fa.html
محرابیون محمدی مرضیه، بابایی زاد ولی اله، رحیمیان حشمت اله، ابراهیم نژاد شاپور. غربالگری چند ژنوتیپ جو در برابر قارچ عامل سفیدک سطحی و بررسی نقش تعدادی از ژنهای مرتبط با بیماریزایی و NH1 در مقاومت به بیماری پژوهشنامه اصلاح گیاهان زراعی 1396; 9 (22) :124-117 10.29252/jcb.9.22.117
1- دانشگاه علوم کشاورزی و منابع طبیعی ساری
2- مرکز تحقیقات کشاورزی و منابع طبیعی مازندران
2- مرکز تحقیقات کشاورزی و منابع طبیعی مازندران
چکیده: (4868 مشاهده)
جو یکی از مهمترین غلات در تغذیه دام بوده که به عنوان منبع جایگزین گندم و برنج در تغذیه رژیمی نیز نقش مهمی دارد. جو همواره در معرض خسارت عوامل متعدد آفات و بیماریها میباشد که سبب کاهش میزان محصول و یا پائین آوردن ارزش غذایی آن میشوند. از جمله عوامل خسارتزا جو قارچ بیوتروف (Bgh) Blumeria graminis f. sp. hordei عامل بیماری سفیدک سطحی است که در برخی مناطق زیر کشت این محصول خسارات جدی وارد میکند. همانند سایر گیاهان، جو نیز با استفاده از سیستمهای دفاعی بر پایه پروتئینها و سایر مواد ضد میکروبی در مقابل بیمارگرها مقاومت میکند و در این میان پروتئینهای مرتبط با بیماری زایی (Pathogenesis- Related Proteins) نقش بسزایی دارند. این تحقیق به منظور بررسی مورفولوژیکی و مولکولی چندین ژنوتیپ جو کشور در تعامل با قارچ Bgh انجام شد. تجزیه وتحلیل آماری نتایج غربالگری نشان داد لاین Mb-86-5 بیشترین حساسیت و بجز ژنوتیپ Beecher، بقیه ژنوتیپهاBIR ، Ceres،Avt/Attiki ،L1242 ، یوسف، نصرت، ماهور،Comp171E ،Rihane-03 در مقایسه با لاین حساسMb-86-5 مقاومت معناداری داشتند. جهت تعیین پروفایل ژنهای دخیل در مقاومت از گیاهچههای یک هفتهایی در ساعات مختلف پس از تلقیح (0-12-24-48 ساعت) نمونه برداری و Total RNA از آنها استخراج شد. نتایج بررسی مولکولی نشان داد که بیان ژنهای PR1، PR5، NH1 و POX به مقدار معنیداری در ژنوتیپ مقاوم در مقایسه با ژنوتیپ حساس افزایش یافت. این نتایج نشان می دهد ژنهای مطالعه شده در مقاومت به بیماریBgh در ژنوتیپ Ceres جو ایفای نقش میکنند.
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