fa نقشه یابی ارتباطی برخی صفات فنولوژیک در جو تحت تنش شوری اصلاح براي تنش هاي زنده و غيرزنده محيطي Special پژوهشي Research <p dir="RTL"><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">پژوهش حاضر در راستای شناسایی نشانگرهای مولکولی مرتبط با صفات فنولوژیک</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">روز تا پنجه &shy;دهی، روز تا ساقه&shy; دهی، روز تا سنبله&shy; دهی، روز از ساقه &shy;دهی تا سنبله&shy; دهی، طول دوره&shy;ی پرشدن دانه و تعداد روز تا رسیدن فیزیولوژیک و با استفاده از 407 نشانگر </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">SSR</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> و </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">AFLP</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> روی 148 ژنوتیپ جو به روش نقشه&shy; یابی ارتباطی انجام شد. این آزمایش در قالب دو طرح آلفا لاتیس با پنج بلوک ناقص در دو تکرار تحت شرایط نرمال و تنش شوری (</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">EC=12 dsm^-1</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">) در سال زراعی 95-1394 و در مزرعه&shy; ی مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی یزد صورت پذیرفت. نقشه &shy;یابی ارتباطی بر اساس مدل خطی مخلوط (</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">MLM</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">) با استفاده از نرم &shy;افزارهای </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">Structure</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> و </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">Tassel</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> انجام شد. براساس 407 نشانگر مورد استفاده در این مطالعه، ساختار ژنتیکی جمعیت به دو زیرجمعیت فرعی (2=</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">K</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">) تقسیم گردید که نتایج حاصل از رسم بارپلات نیز مؤید آن بود. در نقشه&shy; یابی ارتباطی به روش </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">MLM</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">، در شرایط نرمال و تنش شوری به ترتیب 4 و 46</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نشانگر</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ارتباط</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">معنی&shy; داری</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">با</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">صفات</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مورد</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نشان</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">دادند</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تغییرات</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">قابل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">توجهی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">از</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">صفات</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مورد مطالعه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">را</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">توجیه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نمودند</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">.</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">این</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">چندین</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مکان ژنی کمی مشترک</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">برای</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">صفات</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مورد</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شناسایی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">.</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> وجود</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نشانگرهای</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مشترک در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">میان</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">صفات</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">می</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تواند</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ناشی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">از</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">اثرات</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">پلیوتروپی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">یا پیوستگی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نواحی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ژنومی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">دخیل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">کنترل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">این</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">صفات</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">باشد. نتایج</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بدست آمده</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">از</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">حاضر، اطلاعات</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ارزشمندی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">زمینه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مبنای</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ژنتیکی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">صفات مورد</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ارائه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">می &shy;دهد</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">که </span></span></strong>&nbsp;<strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">می&shy;توان</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">برنامه&shy; های اصلاحی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">جو</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">از</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">جمله</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">انتخاب</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">به</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">کمک</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نشانگر</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">استفاده نمود.</span></span></strong><br> &nbsp;<br> &nbsp;</p> <p>Current research was performed to identify molecular markers associated to phonological traits including days to tillering, days to stem elongation, days to heading, days from stem elongation to heading, grain filling period and days to physiological maturity based on 407 AFLP and SSR markers in 148 barley cultivars by association mapping. This experiment was conducted in two alpha lattice designs with five incomplete blocks in two replications under normal and salt stress (EC=12 dsm^-1) conditions in Agriculture and Natural Resources Research and Education Center, Yazd, Iran in 2015-16. Association mapping was evaluated based on mixed linear model (MLM) using Structure and Tassel soft wares. Based on the 407 markers used in this study, population genetic structure subdivided into two subpopulations (K=2) that barplot results also confirmed it. In association mapping based on MLM, 4 and 46 markers showed significant relation with assessed traits under normal and salt stress, respectively and explained considerable variations of studied traits. In this study, some co-localized QTLs were identified for studied traits. Common markers between of traits can be due to pleiotropic effects or linkage between of genomic regions involved in these traits. Results of the current study presented useful information about the genetic basis of the studied traits and can be used in different barley breeding programs including marker-assisted selection.<br> &nbsp;<br> <strong>&nbsp;</strong></p> تنش شوری, جو, ساختار ژنتیکی جمعیت, مدل خطی مخلوط, نقشه­یابی ارتباطی Association mapping, Barley, Mixed linear model, Population genetic structure, Salt stress 12 21 http://jcb.sanru.ac.ir/browse.php?a_code=A-10-664-1&slc_lang=fa&sid=1 mahdiyeh zare kohan مهدیه زارع کهن mahdiyehzare65@gmail.com 10031947532846008081 10031947532846008081 Yes Nadali Babaeian Jelodar نادعلی بابائیان جلودار nbabaeian@yahoo.com 10031947532846008082 10031947532846008082 No reza aghnoum رضا اقنوم reza.aghnoum@gmail.com 10031947532846008083 10031947532846008083 No مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد seyed ali tabatabaee سید علی طباطبایی tabataba4761@ahoo.com 10031947532846008084 10031947532846008084 No seyed kamal Kazemi Tabar سید کمال کاظمی تبار sdklkr@ymail.com 10031947532846008085 10031947532846008085 No