fa مکان یابی ژن های کنترل کننده برخی صفات زراعی گندم نان تحت تنش خشکی با استفاده از نشانگرهای ریزماهواره اصلاح نباتات General پژوهشي Research <div style="text-align: justify;"><strong><span style="color:black;"><span style="font-family:2 mitra;">&nbsp;&nbsp; &nbsp;&nbsp;به&shy; منظور</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">شناسایی</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">ریزماهواره&shy; های</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">پیوسته</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">با</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">برخی صفات زراعی گندم در شرایط تنش خشکی انتهای فصل، جمعیتی شامل 96 خانواده </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">F_2:4</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"> حاصل از تلاقی ژنوتیپ&shy; های خارچیا (والد متحمل) و گاسپارد (والد حساس) طی 2 سال ارزیابی شدند. از میان 92 ریز ماهواره برای ارزیابی والدین، 32 ریزماهواره چند شکلی داشتند که در کروموزوم</span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">های </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">2، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">D</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">2، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">3، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">A</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">4، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">4، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">D</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">4، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">A</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">5، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">5، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">D</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">5، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">6، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">A</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">7، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">7، </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">D</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">7 گندم توزیع شده بودند. بر</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">اساس</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">روش</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">مکان</span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">&shy;</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">یابی</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">فاصله &shy;ای</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">مرکب، سه </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">QTL</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"> برای</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">صفت</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">ارتفاع گیاه مکان&shy;یابی گردید که بر روی کروموزوم </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">7 قرار داشتند و بالاترین </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">QTL</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"> با بیشترین </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">LOD</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"> برابر با 21/3 و در فاصله بین </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">Xgwm274</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"> و </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">Xgwm369</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"> قرار داشت و 3/14 درصد از تغییرات فنوتیپی را توجیه &shy;می کرد، برای صفت تعداد دانه در سنبله اصلی یک </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">QTL</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"> بر روی کروموزوم </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">2 و برای صفت شاخص برداشت نیز یک </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">QTL</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;"> بر روی کروموزوم </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">B</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">4 شناسایی گردید. جایگاه</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">مشترک</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">برخی</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">از </span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">QTL</span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">های</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">مکان&shy;یابی</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">شده</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">بیانگر</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">پیوستگی</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">ژنی</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">یا</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">اثر</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">پلیوتروپیک</span></span></strong> <strong><span style="color:black;"><span style="font-family:2 mitra;">است. اشباع نقشه مورد نظر و ارزیابی پایداری صفات مورد بررسی می &shy;تواند با قابلیت بیشتری شناسایی</span></span></strong><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">QTL </span></span></span></span></strong><strong><span style="color:black;"><span style="font-family:2 mitra;">های کنترل کننده را تأیید نماید و زمینه گزینش بر اساس نشانگر را فراهم نماید.</span></span></strong></div> <div style="text-align: justify;"></div> In order to identify microsatellite markers linked to some traits of wheat under drought stress at the end of the season, the population included 96 family F2:4 genotypes derived from the cross Kharchia (tolerant parent) and Gaspard (susceptible parent) were evaluated for 2 years. Of the 92 microsatellite markers used to assess parents, 32 markers were polymorphic that located on chromosomes 2B, 2D, 3B, 4A, 4B, 4D, 5A, 5B, 5D, 6B, 7A, 7B, 7D. Based on composite interval mapping was found 3 QTL for plant height trait which were located on chromosome 7B and&nbsp; the highest QTL with the largest LOD equal to 3.21 was located between Xgwm274 and Xgwm369 and justified 14.3 percent of phenotypic variation. For number of seeds per main spike trait, 1 QTL was detected on chromosome 2B and 1 QTL was identified on the chromosome 4B for harvest index trait. The common position of some of the located QTLs indicates a genetic linkage or pleiotropic effect. The saturation of the desired map and the evaluation of the stability of the studied traits can confirm the ability to identify the controller QTLs and provide the basis for Marker-Assisted Selection<strong>.</strong><br> &nbsp; تنش خشکی, گندم نان,SSR , QTL Bread Wheat, Drought Stress, QTL, SSR 160 167 http://jcb.sanru.ac.ir/browse.php?a_code=A-10-434-2&slc_lang=fa&sid=1 Jamileh Abedi جمیله عابدی jabedi89@gmail.com 100319475328460011085 100319475328460011085 No Post graduate Student, Graduate University of Advanced Technology دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران Ghasem Mohammadinejad قاسم محمدی نژاد Mohammadinejad@uk.ac.ir 100319475328460011086 100319475328460011086 Yes Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Bahonar University of Kerman, Kerman-Iran گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران Amin Baghizadeh امین باقی زاده 100319475328460011087 100319475328460011087 No Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman-Iran گروه بیوتکنولوژی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان،ایران