Journal of Crop Breeding
پژوهشنامه اصلاح گیاهان زراعی
J Crop Breed
Agriculture
http://jcb.sanru.ac.ir
1
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2228-6128
2676-4628
10.61186/jcb
fa
jalali
1397
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gregorian
2018
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ارزیابی کمی و مولکولی تحمل به تنش خشکی ژنوتیپهای برنج
Relationship between ISSR and SSR Markers Indicators of Drought Tolerance in Rice Genotypes
اصلاح براي تنش هاي زنده و غيرزنده محيطي
Special
پژوهشي
Research
<p> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">اصلاح</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">برنج</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">برای</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تحمل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">به</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">خشکی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نیازمند</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ارزیابی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">صحیح</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تنوع</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">موجود</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">جمعیت­ های اصلاحی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تجزیه</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ژنتیکی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> شاخص ­های مرتبط</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">با</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تحمل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">به</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">خشکی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">است. به­ منظور</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بررسی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شاخص ­های</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تحمل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">به</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تنش</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شناسایی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ژنوتیپ­ های</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">متحمل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">به</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شرایط</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تنش خشکی،</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">59</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ژنوتیپ</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">برنج</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">قالب</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">طرح</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بلوک­های</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">کامل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تصادفی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">با 3</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تکرار</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">دو</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شرایط</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">غرقاب</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تنش خشکی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در سال</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">زراعی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">92 مورد</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">آزمایش</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">قرار</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">گرفتند</span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">. </span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در این تحقیق ارتباط بین شاخص ­ها و نشانگر­های مولکولی در برنج با استفاده از 10 شاخص و 189 مکان ژنی حاصل از 36 جفت نشانگر</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> SSR </span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و 144 مکان ژنی حاصل از 10 جفت نشانگر </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> ISSR</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بر روی 59 ژنوتیپ برنج در شرایط غرقاب و تنش خشکی مورد مطالعه قرار گرفت.</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تحلیل</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">همبستگی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بین</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">عملکرد</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شرایط غرقاب</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و تنش خشکی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شاخص</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تحمل به</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">خشکی نشان</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">داد</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">که شاخص­های </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">SSI</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> STI</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> MP</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> GMP</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> HM</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> YI</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">را</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">به­ عنوان</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شاخص­هایی برتر</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">در</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">هر</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">دو</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">شرایط</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">غرقاب</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تنش خشکی</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">که قادرند</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">ژنوتیپ ­های مقاوم</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">با</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">عملکرد</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">بالا</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">را</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نشان</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">دهند</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">انتخاب</span></span></strong> <strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">نمود. براساس تجزیه رگرسیون گام­ به­ گام داده ­های مولکولی ریزماهواره و بین ریزماهواره با شاخص­ ها، در مجموع به ترتیب 57 و 44 نشانگر آگاهی بخش با شاخص ­ها شناسایی شد. بیشترین توجیه تغییرات بر اساس داده­های ریزماهواره مربوط به</span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> شاخص تحمل به تنش (</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">STI</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">) </span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">با 541/0 توسط مکان­های ژنی</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">RM5424-E </span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">RM462-B </span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">RM549-E </span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">RM8206-C </span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">RM7118-B </span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">RM5424-D </span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">، </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">RM3294-B</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> RM6324-B</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">، </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> RM5780-G</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">تبین شد </span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">و</span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> بیشترین توجیه تغییرات براساس داده­ های بین ریزماهواره مربوط به</span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> شاخص عملکرد(</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">YI</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">) </span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">با 469/0 توسط<br>
مکان­های ژنی</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">ISSR3-1</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">، </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">ISSR1-6</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">، </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">ISSR2-8</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">،</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">ISSR9-1</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">، </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;">ISSR10-8</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;">، </span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman bold,serif;"><span style="font-size:8.0pt;"> ISSR10-10</span></span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> بود.</span></span></strong><strong><span style="font-family:2 mitra;"><span style="font-size:10.0pt;"> از نتایج این تحقیق در تامین اطلاعات اولیه برای انتخاب غیر­مستقیم صفات مفید از طریق نشانگر­های مولکولی می­باشد.</span></span></strong><br>
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</p>
Breeding of rice for drought tolerance requires proper evaluation and analysis of genetic diversity in breeding populations related to drought tolerance<span dir="RTL">.</span> In order to identify indices for tolerant and sensitive rice genotypes to drought stress, an experiment with 59 genotypes of rice was conducted based of a complete block design with three replications in non<span dir="RTL">-</span>stressed (flooding) and stressed conditions in 2013<span dir="RTL">.</span> Analysis of correlations between the performance of flooding and drought stress tolerance index showed that the indices SSI, STI, MP, GMP, HM and YI as are superior indices for both flooding and drought conditions. Stepwise regression analysis between microsatellite and microsatellite molecular data with indices, a total of 57 and 44, informative markers were identified respectively<span dir="RTL">.</span> The most variations based on microsatellite data on stress tolerance index (STI) was explained with 0.541 by gene loci of RM5424-E, RM462-B, RM549-E, RM8206-C, RM7118-B, RM5424-D, RM3294-B, RM6324-B, RM5780-G and the most variations between microsatellite data based on the performance index (YI) was explained with 0.469 by gene loci of ISSR3-1, ISSR1-6, ISSR2- 8, ISSR9-1, ISSR10-8, ISSR10-10. Results of present study provided a few preliminary data for indirect selection of useful traits using molecular markers.<br>
<br>
ببرنج, رگرسیون گامبهگام, شاخصها تحمل به تش, SSR, ISSR
ISSR, Rice, Stepwise regression, SSR, Tolerance Indices
195
206
http://jcb.sanru.ac.ir/browse.php?a_code=A-10-363-2&slc_lang=fa&sid=1
reza
karim koshte
رضا
کریم کشته
rezakarim597@yahoo.com
10031947532846008151
10031947532846008151
Yes
Hossien
sabouri
حسین
صبوری
hos.sabouri@gmail.com
10031947532846008152
10031947532846008152
No