Volume 9, Issue 24 (3-2018)                   jcb 2018, 9(24): 95-102 | Back to browse issues page


XML Persian Abstract Print


Department of Agriculture, Payam Noor University
Abstract:   (4052 Views)
Fennel (Foeniculum vulgare Mill.) is one of the well-known medicinal and aromatic plants. To determine variability between 16 Iranian Fennel landrace based on molecular markers, this experiment was conducted in the biotechnology laboratory of Payame Noor University of Kermanshah in 2014. In this experiment, 16 landrace of the Foeniculum vulgare Mill. were selected from the Natural Resources Gene Bank of Iran of Research Institute of Forest and Rangeland. Landrace were evaluated based on 15 SCoT primers markers. All of the SCot primers showed 77 visible bands in which four bands were similar patterns. The SC5 and SC29 primers had the most number of bands with 10 and 9 bands respectively, while SC10, SC26 and SC44 with two bands showed the least band numbers. The Polymorphic information content (PIC), marker index (MI), EMR and RP indices were calculated for all primers. With this point of view, SC15 and SC63 were the best primers to identify variability among these Fennels. Total genetic similarity based on these primers was 66 percent. The greatest genetic similarity was between Ardabil1 with Ardabil2 and Tehran2 landrace. The lowest genetic distance was between Ghazvin and Yazd fennel landrace. Cluster analysis based Jakard coefficient by UPGMA was classified all genotype to four groups and this clustering was confirmed by principal coordinate (PCo) and analysis of molecular variance (AMOVA). Portion of between group variance was just 24 percent of total variance.   
Full-Text [PDF 453 kb]   (3343 Downloads)    
Type of Study: Research | Subject: اصلاح نباتات، بیومتری
Received: 2018/03/10 | Revised: 2019/04/14 | Accepted: 2018/03/10 | Published: 2018/03/10

References
1. Altıntas, S., F. Toklu, S. Kafkas, B. Kilian, A. Brandolini and H.O. Zkan. 2008. Estimating Genetic Diversity in Durum and Bread Wheat Cultivars from Turkey Using AFLP and SAMPL Markers. Plant Breeding, 127: 9-14.
2. Bahmani, K., A. Izadi darbandi and R. Baghchghi. 2012. The study of genetic variation Iranian fennel using RAPD molecular marker. Fourteenth Iranian Genetics Congress, 12: 15-20.
3. Davis, TM., H. Yu. Haigis and P.J. McGowan. 1995. Template mixing: a method of enhancingdetection and interpretation of codominant RAPD markers. Theoretical and Applied Genetics, 91: 582-8 [DOI:10.1007/BF00223283]
4. Hasani, M.H., S. Torabi, M. Omidi, A.R. Etminan and T. Dastmalchi. 2011. The study of genetic variation Foeniculum vulgar Mill. Using AFLP molecular marker. Iranian Journal of Field Crop Science, 42: 597-604.
5. Hou, Y., Z. Yan and Y. Wei. 2005. Genetic diversity in barely from west China based on RAPD and ISSR analysis Barely. Genetics Newsletter, 35: 9-22.
6. Hu, J. and BA. Vick. 2003. Target region amplification polymorphism: a novel marker technique forplant genotyping. Plant Molecular Biology Reporter, 21: 289-94. [DOI:10.1007/BF02772804]
7. Kalendar, R. 2007. FastPCR: a PCR primer design and repeat sequence searching software with additional tools for the manipulation and analysis of DNA and protein. Available at www.biocenter.helsinki.fi/programs/fastpcr.htm, Genes, Genomes and Genomics, Special Issue 1: 1-14.
8. Kochaki. A., M. Nasiri and F. Najafi. 2004. The agro biodiversity of medical and aromatic plants in Iran, 2: 209-214.
9. Kumar, M., G.P. Mishra, R. Singh, J. Kumar, P.K. Naik and Sh.B. Singh. 2009. Correspondence of ISSR and RAPD Markers for Comparative Analysis of Genetic Diversity among Different Apricot Genotypes from Cold Arid Deserts of Trans-Himalayas. Physiology and Molecular Biology of Plants, 15: 225-236. [DOI:10.1007/s12298-009-0026-6]
10. Mahjoob, B., H. Najafi-Zarini and S.HR. Hashemi. 2014. Assessment of Genetic Relationships among 36 Brassica Genotypes using ISSR Molecular Markers. Journal of Crop Breeding, 6: 96-106.
11. Mardi, M., A. Taleei and M. Omidi. 2003. Study on genetic variation and yield Components Indies type chickpea. Iranian Journal of Agricultural Science, 34: 345-351.
12. Mehdikhani, H., S. Mahmood and H. Zeinali. 2013. Study of Genetic Diversity in Chamomile Landraces (Matricaria aurea (Loefl.) Sch. Bip.) Using Random and Semi-Random Primers. Journal of Crop Breeding, 5: 69-82.
13. Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and A. Rafalski. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238. [DOI:10.1007/BF00564200]
14. Safayi, M., S. Jafarnia and S. Khosroshahi. 2008. The most important medical plants of the world, 222-223
15. Salehi Jozani, G., S. Abd- Mishani, A.H. Hoseinzadeh and B.E .Seied Tabatabaei. 2003. Genetic diversity analysis of commercial potato cultivars (solanum tuberosum ) in iran using RAPD-PCR technique. Iranian Journal of Agricultural sciences, 34: 1021-1029.
16. Shabanian, N., L. Alikhani and M.S. Rahmani. 2015. Phenotypic and genotypic diversity in rant oak (Quercus brantii) populations of declining north-Zagros forests using biochemical characteristics and molecular SCoT marker. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 23: 23-29
17. Torres, A.M., NF. Weeden and A. Martin. 1993. Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theoretical and Applied Genetics, 85: 935-945. [DOI:10.1007/BF00215032]
18. Yazdani, D., S. Shahnazi and H. Seifi. 2004. Cultivation of Medical Plants. Applied guide for cultivation of 40 important medical plants in Iran, 1: 73-75.
19. Zahid, N.Y., N.A. Abbasi, I.A. Hafiz and Z. Ahmad. 2009. Genetic diversity of indigenous fennel, (Foeniculum vulgare). Germplasm in Pakistan assessed By RAPD markers. Pakistan Journal of Botany, 41: 1759-1767.

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.