دوره 9، شماره 22 - ( تابستان 1396 )                   جلد 9 شماره 22 صفحات 117-124 | برگشت به فهرست نسخه ها

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جو یکی از مهم­ترین غلات در تغذیه دام بوده که به عنوان منبع جایگزین گندم و برنج در تغذیه رژیمی نیز نقش مهمی دارد. جو همواره در معرض خسارت عوامل متعدد آفات و بیماری­ها می­باشد که سبب کاهش میزان محصول و یا پائین آوردن ارزش غذایی آن می­شوند. از جمله عوامل خسارتزا جو قارچ بیوتروف (Bgh) Blumeria graminis f. sp. hordei عامل بیماری سفیدک سطحی است که در برخی مناطق زیر کشت این محصول خسارات جدی وارد می­کند. همانند سایر گیاهان، جو نیز با استفاده از سیستم­های دفاعی بر پایه پروتئین­ها و سایر مواد ضد میکروبی در مقابل بیمارگرها مقاومت می­کند و در این میان پروتئین­­های مرتبط با بیماری زایی (Pathogenesis- Related Proteins) نقش بسزایی دارند. این تحقیق به منظور بررسی مورفولوژیکی و مولکولی چندین ژنوتیپ جو کشور در تعامل با قارچ Bgh انجام شد. تجزیه وتحلیل آماری نتایج غربالگری نشان داد لاین Mb-86-5 بیشترین حساسیت و بجز ژنوتیپ Beecher، بقیه ژنوتیپ‏هاBIR ، Ceres،Avt/Attiki ،L1242 ، یوسف، نصرت، ماهور،Comp171E ،Rihane-03 در مقایسه با لاین حساسMb-86-5 مقاومت معنا­داری داشتند. جهت تعیین پروفایل ژن­های دخیل در مقاومت از گیاهچه­های یک هفته­ایی در ساعات مختلف پس از تلقیح (0-12-24-48 ساعت) نمونه برداری و Total RNA از آنها استخراج شد. نتایج بررسی مولکولی نشان داد که بیان ژن­های PR1، PR5، NH1 و POX به مقدار معنی­داری در ژنوتیپ مقاوم­ در مقایسه با ژنوتیپ حساس افزایش یافت. این نتایج نشان می دهد ژن­های مطالعه شده در مقاومت به بیماریBgh  در ژنوتیپ Ceres جو ایفای نقش می­کنند.
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نوع مطالعه: پژوهشي | موضوع مقاله: اصلاح نباتات، بیومتری

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