دوره 10، شماره 28 - ( زمستان97 1397 )
جلد 10 شماره 28 صفحات 37-27 |
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دانشگاه زابل
چکیده: (2845 مشاهده)
تربچه از تیره شب بویان، دارای موادی از جمله سنوول، مالوین کلراید، ساپوژنین، سولفورافان و رافانین بوده و از خواص درمانی متعددی برخوردار است. در تحقیق حاضر تنوع ژنتیکی 7 جمعیت تجاری/بومی گـیاه دارویی تربچه با استفاده از نشانگر رتروترانسپوزونی IRAP بررسی شد. آغازگر ترکیبی LTR2 & LTR15 با 1 باند کمترین تعداد و آغازگر LTR23 با 16 باند بیشترین تعداد را در میان باندهای تولیدی توسط هر آغازگر داشتند. میانگین تعداد باند در کل جایگاه ها برابر 93/6 بود. بیشترین میزان شاخص چندشکلی (41/0) مربوط به آغازگر Sukkula و کمترین میزان شاخص چندشکلی (18/0) مربوط به آغازگر LTR2- LTR15 و با میانگین کل 28/0 مشاهده گردید. بیشترین میزان شاخص نشانگری (1/4) مربوط به آغازگر LTR3 و کمترین میزان شاخص نشانگری (18/0) مربوط به ترکیب آغازگری LTR15 & LTR2 مشاهده گردید. بیشترین میزان تعداد باند مؤثره، شاخص تنوع شانن و شاخص تنوع نی به ترتیب 82/1، 64/0 و 45/0 متعلق به آغازگر Sukkula و کمترین میزان تعداد باند مؤثره، شاخص تنوع شانن و شاخص تنوع نی به ترتیب 18/1، 27/0 و 15/0 متعلق به آغازگــر rtdk بود. در بیــن جمعیت های تربچه بیشترین درصد مکان چندشکل، تعـداد باند مؤثره، شاخـص تنـوع نی و شاخص تنوع شانن به تـرتیـب 85/46، 05/1، 17/0 و 26/0 متعلق به جمعیت Profit بود. تغییرات درون و بین جمعیت‌ها با استفاده از تجزیه واریانس مولکولی نشان داد که 59 درصد کل تغییرات ژنتیکی به تنوع درون جمعیت‌ها اختصاص داشت. نتایج این تحقیق نشان داد که می توان از جمعیت Profit (چون دارای بیشترین درصد چندشکلی و تنوع شانن و نی و تعداد باند موثره بوده) به‌عنوان یکی از پایه‌های پدری یا مادری و یا دهنده ژن انتخاب در اصلاح تربچه های ایران استفاده کرد.
نوع مطالعه: پژوهشي |
موضوع مقاله:
بيوتكنولوژي گياهي
دریافت: 1396/5/21 | ویرایش نهایی: 1398/2/18 | پذیرش: 1397/1/14 | انتشار: 1397/12/11
دریافت: 1396/5/21 | ویرایش نهایی: 1398/2/18 | پذیرش: 1397/1/14 | انتشار: 1397/12/11
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