Volume 10, Issue 26 (9-2018)                   jcb 2018, 10(26): 153-165 | Back to browse issues page


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Razi University
Abstract:   (3875 Views)

Intersimple sequence repeat (ISSR) markers were evaluated in order to identify informative markers associated with drought tolerance indices in bread wheat (Triticum aestivum L.) genotypes. Eighteen ISSR primers amplified 92 loci among 20 bread wheat genotypes. Polymorphic information content (PIC) ranged from 0.46 (UBC-857, UBC-864, UBC-867, is9) to 0.21 (is7), with an average of 2.05. Stepwise regression analysis between molecular data as independent variable, and parametric stability statistics as dependent variables was performed to identify informative markers associated with the parametric stability statistics. Most of the used ISSR primers showed significant association with the parametric stability statistics. Stability statistics included Finlay and Wilkinson’s coefficient of regression, Perkins and Jinks’s coefficient of regression, MBIW, Lin and Binns’s superiority index, SFi and NP (2)i were explained by more primers. ISSR markers, UBC-848, UBC-869 and is5 showed the most association with stability statistics. It is possible to use these markers along with stability statistics in wheat breeding programs for identification of stable genotypes and suitable parents to produce mapping populations. Also, these results could be useful in marker- assisted breeding programs when no other genetic information is available. Some of ISSR markers were associated with more than one trait in multiple regression analysis. Such an association may arise due to pleiotropic effect of the linked quantitative trait locus (QTL) on different traits.
  

 

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Type of Study: Research | Subject: General
Received: 2016/07/5 | Revised: 2018/09/26 | Accepted: 2017/07/1 | Published: 2018/09/26

References
1. Ahangari, A., M. Rasoli and M. Naderi. 2009. Evaluation of effective traits in drought stress resistance in wheat. Agricultural Proceedings Agricultural and Plant Breeding, Markazi Province Agricultural Jihad Organization (In Persian).
2. Abdollahi Mandolkani, B. and H. Azizi. 2014. Association analysis of morphologic traits with by inter-simple sequence repeat markers in Alfalfa (Medicago sativa L.). Journal of Cellular and Molecular Research, 27(2): 260-268 (In Persian).
3. Azizi, H., A., Bernosi, B. Abdollahi Mandolkani and R. Darvish zadeh. 2011. Study of genetic diversity and structure of Alfalfa(Medicago sativa L.) populations using the inter-simple sequence repeat markers. New Genealogy Journal, 6(4): 61-69 (In Persian).
4. Ashraf, M. and P.J. Harris 2005. Abiotic stresses: plant resistance through breeding and molecular approaches. Food Products Press. USA. Binghamton, 725 pp.
5. Briggle, L. and B. Curtis. 1987. Wheat worldwide. Wheat and wheat improvement. 1-32.
6. Gebhardt, C., A. Ballvora, B. Walkemeier, P. Oberhagemann and K. Schüler. 2004. Assessing genetic potential in germplasm collections of crop plants by marker-trait association: a case study for potatoes with quantitative variation of resistance to late blight and maturity type. Molecular Breeding, 13: 93-102. [DOI:10.1023/B:MOLB.0000012878.89855.df]
7. Carvalho, A., M. Matos, J. Lima-Brito, H. Guedes-Pinto and C. Benito. 2005. DNA fingerprint of F1 interspecific hybrids from the Triticeae tribe using ISSRs. Euphytica, 143: 93-99. [DOI:10.1007/s10681-005-2839-x]
8. Culp, T., D. Harrell and T. Kerr. 1979. Some genetic implications in the transfer of high fiber strength genes to upland cotton. Crop Science, 19: 481-484. [DOI:10.2135/cropsci1979.0011183X001900040013x]
9. Ebrahimi, A., M. Naghavi, M. Sabokdast and S.S.A. Moradi. 2011. Association analysis of agronomic traits with microsatellite markers in Iranian barley landraces barley. Modern Genetics Journal, 6(1): 35-43.
10. Gomez, K.A. and A.A. Gomez. 1984. Statistical procedures for agricultural research. John Wiley and Sons. Science, 680 pp.
11. Huang, J. and M. Sun. 2000. Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA. Theoretical and Applied Genetics, 100: 1050-1060. [DOI:10.1007/s001220051386]
12. Inostroza, L., A. del Pozo, I. Matus, D. Castillo, P. Hayes, S. Machado and A. Corey. 2009. Association mapping of plant height, yield and yield stability in recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. Molecular Breeding, 23: 365-376. [DOI:10.1007/s11032-008-9239-6]
13. Jabbarzadeh, Z., M. Khosh-Khui, H. Salehi and A. Saberivand .2013. Inter simple sequence repeat (ISSR) markers as reproducible and specific tools for genetic diversity analysis of rose species. African Journal of Biotechnology, 9: 6091-6095.
14. Kar, P.K., P.P. Srivastava, A.K. Awasthi and S.R. Urs. 2008. Genetic variability and association of ISSR markers with some biochemical traits in mulberry (Morus spp.) genetic resources available in India. Tree Genetics and Genomes. 4: 75-83 [DOI:10.1007/s11295-007-0089-x]
15. Khaled, A.G.A., M.H. Motawea and A.A. Said. 2015. Identification of ISSR and RAPD markers linked to yield traits in bread wheatunder normal and drought conditions. Journal of Genetic Engineering and Biotechnology. [DOI:10.1016/j.jgeb.2015.05.001]
16. Kraakman A.T., R.E. Niks P.M. Van den Berg P. Stam and F.A. Van Eeuwijk. 2004. Linkage disequilibrium mapping of yield and yield stability in modern spring barley cultivars. Genetics, 168: 435-446. [DOI:10.1534/genetics.104.026831]
17. Li, Q., Q.C. Liu, H. Zhai, D.F. MA, X. Wang, X.Q. Li and Y.P. Wang. 2008. Genetic diversity in main parents of sweetpotato in China as revealed by ISSR markers. Acta Agronomica Sinica, 34: 972-977. [DOI:10.1016/S1875-2780(08)60036-X]
18. Meredith, W.R. and R. Bridge. 1971. Breakup of linkage blocks in cotton, Gossypium hirsutum L. Crop Science, 11: 695-698. [DOI:10.2135/cropsci1971.0011183X001100050027x]
19. Miletic, R., M. Zikic, N. Mitic and R. Nicolic. 2005. Pomological characteristic of superior selections of European filbert (C. avellana L.). Genetica. 37: 103-111. [DOI:10.2298/GENSR0502103M]
20. Motawea, M., A. Said and A. Khaled. 2015. ISSR Markers-Trait Associations and Stability. Plant Breeding Biotechnology, 3(2): 167-177. [DOI:10.9787/PBB.2015.3.2.167]
21. Mohammadi M., R. Karimizadeh, N. Sabaghnia and M.K. Shefazadeh. 2012a. Genotype -Environment Interaction and Yield Stability Analysis of New Improved Bread Wheat Genotypes. Turkish Journal of Field Crops, 17: 67-73.
22. Najaphy, A., R.A. Parchin and E. Farshadfar. 2012. Comparison of phenotypic and molecular characterizations of some important wheat cultivars and advanced breeding lines. Australian Journal of Crop Science, 6: 326.
23. Pritchard, J.K., M. Stephens and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genetics, 155: 945-959.
24. Razeghi yadak, F., SH. Haidari and K. Sori. 2010. Effect of drought stress on activity of acid and alkaline phosphatase in seed embryonic axis of two bread wheat cultivars in early stages of germination. Journal of Iranian Crop Science, 41(2): 385-393 (In Persian).
25. Rakshit S., K. Ganapathy, S. Gomashe, A. Rathore, R. Ghorade, M.N. Kumar, K. Ganesmurthy, S. Jain, M. Kamtar and J. Sachan. 2012. GGE biplot analysis to evaluate genotype, environment and their interactions in sorghum multi-location data. Euphytica, 185: 465-479. [DOI:10.1007/s10681-012-0648-6]
26. Rosegrant, M. and M. Agcaoili. 2010. Global food demand, supply, and price prospects to 2010. International Food Policy Research Institute, Washington, DC USA.
27. Semagn, K., Å. Bjørnstad and M. Ndjiondjop. 2006. An overview of molecular marker methods for plants. African Journal of Biotechnology, 5(25): 2540-2568.
28. Virk, P., B. Ford-Lloyd, M. Jackson, H. Pooni, T. Clemeno and H. Newburry. 1996. Marker-assisted prediction of agronomic traits using diverse rice germplasm. 1995. Third International Rice Genetics Symposium, Manila (Philippines). International Rice Research Institute.
29. Vaillancourt, A., K. Nkongolo, P. Michael and M. Mehes. 2008. Identification, characterisation, and chromosome locations of rye and wheat specific ISSR and SCAR markers useful for breeding purposes. Euphytica, 159(3): 297-306. [DOI:10.1007/s10681-007-9492-5]
30. Wang, L.X., H.B. Li, T.C. Gu, L.H. Liu, B.S. Pang, J. Qiu and C.P. Zhao. 2014. Assessment of wheat variety stability using SSR markers. Euphytica, 195(3): 435-452. [DOI:10.1007/s10681-013-1006-z]
31. Wolff, K. and M. Morgan-Richards. 1998. PCR markers distinguish Plantago major subspecies. Theoretical and Applied Genetics, 96(2): 282-286. [DOI:10.1007/s001220050737]
32. Zietkiewicz, E., A. Rafalski and D. Labuda. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20(2): 176-183. [DOI:10.1006/geno.1994.1151]

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